Internal control nucleic acids for amplification
Nucleic acids sequences that can be used for nucleic acid amplification, for example quantitative nucleic acid amplification, are provided herein.
1. A composition comprising:
a polynucleotide comprising a sequence at least 97% identical to the sequence of SEQ ID NO: 1;
a forward primer comprising an oligonucleotide at least 85% identical to SEQ ID NO: 3 or 5;
a reverse primer comprising an oligonucleotide at least 85% identical to SEQ ID NO: 4 or 6;
a single-stranded oligonucleotide probe comprising a detectable moiety attached thereto and a sequence at least 85% identical to SEQ ID NO: 2; and
a sample, wherein the sample does not comprise genomic DNA of the genus Drosophila.
2. The composition of claim 1 , wherein the forward primer is at least 95% identical to SEQ ID NO: 3.
3. The composition of claim 1 , wherein the reverse primer is at least 95% identical to SEQ ID NO: 4.
4. The composition of claim 1 , wherein the forward primer comprises SEQ ID NO: 3, and the reverse primer comprises SEQ ID NO: 4.
5. The composition of claim 1 , wherein the forward primer comprises SEQ ID NO: 5, and the reverse primer comprises SEQ ID NO: 6.
6. The composition of claim 1 , wherein the single-stranded oligonucleotide probe comprises a sequence at least 95% identical to SEQ ID NO: 2.
7. The composition of claim 1 , wherein the single-stranded oligonucleotide probe comprises SEQ ID NO: 2.
8. The composition of claim 1 , wherein the single-stranded oligonucleotide probe further comprises at least one fluorophore, and at least one quencher.
9. The composition of claim 1 , wherein the forward primer is at least 95% identical to SEQ ID NO: 5.
10. The composition of claim 1 , wherein the reverse primer is at least 95% identical to SEQ ID NO: 6.
11. The composition of claim 1 , wherein the sequence of the polynucleotide is not genomic DNA sequence of an organism of the genus Drosophila.
12. A kit comprising:
an isolated nucleic acid template for nucleic acid amplification comprising a sequence having at least 97% identity to SEQ ID NO 1;
a first primer comprising an oligonucleotide having at least 85% identity to SEQ ID NO: 3 or 5;
a second primer comprising an oligonucleotide having at least 85% identity to SEQ ID NO: 4 or: 6; and
an oligonucleotide probe comprising a detectable moiety attached thereto and an oligonucleotide comprising a sequence at least 85% identical to SEQ ID NO: 2.
13. The kit of claim 12 , wherein the first primer comprises an oligonucleotide having at least 95% identity to SEQ ID NO: 3 or 5.
14. The kit of claim 12 , wherein the second primer comprises an oligonucleotide having at least 95% identity to SEQ ID NO: 4 or 6.
15. The kit of claim 12 , wherein the first primer comprises SEQ ID NO: 3 or 5.
16. The kit of claim 12 , wherein the second primer comprises SEQ ID NO: 4 or 6.
17. The kit of claim 12 , wherein the first primer comprises SEQ ID NO: 3 and the second primer comprises SEQ ID NO: 4, or wherein the first primer comprises SEQ ID NO: 5 and the second primer SEQ ID NO: 6.
18. The kit of claim 17 , wherein the oligonucleotide probe comprises SEQ ID NO: 2.
19. The kit of claim 12 , wherein the oligonucleotide probe comprises SEQ ID NO: 2.
20. An isolated nucleic acid that comprises SEQ ID NO: 1.