Regenerative cell and adipose-derived stem cell processing system and method
An adipose-derived stem cell (ASC), regenerative cell and/or regenerative factor processing system including a tissue extraction device for extracting raw tissue, such as adipose tissue, from a patient, an ASC, regenerative cell and/or regenerative factor isolator, and an implantation device for re-introducing the isolated ASCs, regenerative cells and/or regenerative factors into the patient.
1. An adipose-derived stem cell processing device comprising:
a first chamber for receiving and containing raw tissue;
an ultrasonic generator configured with the first chamber and configured to induce liquefaction of the raw tissue by exciting the raw tissue at sub-cavitation energy-levels and at the resonant frequency of cells, cellular structures, cell clusters, collagen fibrils and/or extracellular matrix components;
a second chamber that is fluidly-coupled to the first chamber via a valve; and
a filter configured between the first chamber and the second chamber.
2. The processing device of claim 1 , wherein the first chamber comprises a bag.
3. The processing device of claim 1 , wherein the raw tissue is received using negative pressure provided by a vacuum source.
4. The processing device of claim 1 , wherein the raw tissue is received from a cannula.
5. An adipose-derived stem cell processing method comprising:
extracting raw tissue from a patient and passing it to the device of claim 1 ;
inducing liquefaction of the raw tissue by exciting it with ultrasonic energy at sub-cavitation energy-levels and at the resonant frequency of cells, cellular structures, cell clusters, collagen fibrils and/or extracellular matrix components; and
isolating ASCs from the raw tissue.
6. The method of claim 5 , further comprising dissociating the stem cells by performing at least one of: filtering using a sieve, hypoosmotic lysis targeting of erythrocytes and/or adipocytes, introduction of hyperosmotic or hypoosmotic agents to induce dissociation of collagen, lowering the temperature of the raw tissue, increasing the internal pressure of the raw tissue, providing vortex and agitation, lowering or raising the osmotic pressure of the suspension or tissue, lowering or raising the pH level of the raw tissue, or causing mechanical disruption using blades.
7. The method of claim 5 , wherein the first chamber comprises a bag and pressure is applied to the bag during the excitation of the raw tissue.
8. The method of claim 5 , further comprising cycling through a range of durations, frequencies and amplitudes according to the size of the cells, cellular structures, cell clusters, collagen fibrils and/or extracellular matrix components.
9. The processing system of claim 1 , further comprising one or more dissociation enhancement devices configured to enhance dissociation of the stem cells from the raw tissue, wherein the one or more dissociation enhancement devices are configured to perform at least one of: filtering using a sieve, hypo-osmotic targeting of erythrocytes and/or adipocytes, lowering the temperature of the raw tissue, increasing the internal pressure of the raw tissue, providing vortex and agitation, lowering or raising the osmotic pressure of the suspension or raw tissue, lowering or raising the pH level of the raw tissue, or causing mechanical disruption using blades.
10. The processing system of claim 1 , wherein the raw tissue comprises adipose tissue.
11. The processing system of claim 1 , wherein the ultrasonic generator is configured to cycle through a range of durations, frequencies and amplitudes according to the size of the cells, cellular structures, cell clusters, collagen fibrils and/or extracellular matrix components.
12. The processing system of claim 1 , wherein the valve is configured with a luer lock or port.