Efficient process for producing saccharides and ethanol from a biomass feedstock
Described herein is a process for producing saccharides and ethanol from biomass feedstock that includes (a) producing an enzyme composition by culturing a fungal strain(s) in the presence of a lignocellulosic medium, (b) using the enzyme composition to saccharify the biomass feedstock, and (c) fermenting the saccharified biomass feedstock to produce ethanol. The process is scalable and, in certain aspects, is capable of being deployed on farms, thereby allowing local production of saccharides and ethanol and resulting in a reduction of energy and other costs for farm operators. Optional steps to improve the biomass-to-fuel conversion efficiency are also contemplated, as are uses for byproducts of the process described herein.
1. A spent biomaterial produced by the method comprising
a. culturing a fungal strain comprising Trichoderma SG2 , Trichoderma SG4, or a combination thereof, in culture medium, in the presence of a lignocellulosic medium and a starch to produce an enzyme composition and a spent biomaterial; and
b. separating the spent biomaterial from the enzyme composition.
2. The biomaterial of claim 1 , wherein when the fungal strain is Trichoderma SG2 or SG4 and the enzyme composition comprises cellulase, xylanase, beta-glucosidase, beta-xylosidase, amylase, or any combination thereof.
3. The biomaterial of claim 1 , wherein the starch comprises an expired food product selected from the group consisting of corn starch, potato starch, rice starch, tapioca starch, bread or other bakery products, bread waste, bakery waste, donuts, donut waste, and any combination thereof.
4. The biomaterial of claim 1 , wherein the enzyme composition is separated from the spent material by filtration or centrifugation.
5. The biomaterial of claim 1 , wherein the biomaterial comprises Trichoderma SG2 , Trichoderma SG4, or a combination thereof.
6. The composition of claim 1 , wherein Trichoderma SG2 , Trichoderma SG4, or a combination thereof is cultured in the presence of a lignocellulosic medium and a starch.
7. An animal feed comprising the spent biomaterial of claim 1 .
8. A composition for improving plant health comprising the biomaterial of claim 1 .
9. A method for remediating soil comprising applying the spent biomaterial of claim 1 to the soil.
10. An enzyme composition produced by culturing a fungal strain comprising Trichoderma SG2 , Trichoderma SG4, or a combination thereof, in the presence of a lignocellulosic medium and a starch.
11. The composition of claim 10 , wherein when the fungal strain is Trichoderma SG2 or SG4 and the enzyme composition comprises cellulase, xylanase, beta-glucosidase, beta-xylosidase, amylase, or any combination thereof.
12. The composition of claim 10 , wherein the starch comprises an expired food product selected from the group consisting of corn starch, potato starch, rice starch, tapioca starch, bread or other bakery products, bread waste, bakery waste, donuts, donut waste, or any combination thereof.