Method for diagnosis of diseases using morphological characteristics of luterial
The present invention relates to a method for diagnosis of diseases using morphological characteristics of luterial which exists in blood. According to the present invention, morphological characteristics of luterial, such as the number, size or shape, and movement (nano-tracking speed) thereof change according to the kind and progress of a disease, and thus diagnosis and prognosis of a disease (particularly, cancer) can be effectively determined by observing and measuring the characteristics of luterial in the blood.
1. A method of treating a cancer patient, said method comprising:
(a) imaging luterial from a sample of a body fluid of the patient, by microscopy, to present luterial shape(s), wherein the patient has a cancer selected from the group consisting of lung cancer, breast cancer, pancreatic cancer, common bile duct cancer, pleural mesothelioma thyroid cancer, ovarian cancer, biliary track cancer, prostate cancer, acute lymphoblastic leukemia, liver cancer, angiosarcoma of liver, colorectal cancer, uterine cancer, gastric cancer, rectal cancer, acute myeloid leukemia, rectal cancer, and non-small cell lung cancer;
(b) based on the luterial shape(s) in the luterial imaged by microscopy from the sample of the body fluid of the patient, diagnosing a progressionary stage of said cancer; and
(c) administering to the cancer patient a cancer treatment based on the progressionary stage of said cancer that is diagnosed in step (b).
2. The method of claim 1 , wherein the luterial shape(s) on which said diagnosing is based comprises one or more shapes selected from the group consisting of rod shapes, rod-mass shapes, mass-rod shapes, mass shapes, and complex shapes.
3. The method of claim 1 , wherein said imaging, by microscopy, is conducted with an optical microscope, confocal laser scanning microscope, or electron microscope.
4. The method of claim 1 , wherein said imaging, by microscopy, is conducted with a dark field microscope.
5. The method of claim 1 , wherein said imaging, by microscopy, comprises microscopic observation of the luterial from the sample of the body fluid of the patient.
6. The method of claim 1 , wherein said imaging, by microscopy, comprises photographing the luterial from the sample of the body fluid of the patient.
7. The method of claim 1 , wherein the body fluid of the patient is blood, saliva, lymph, semen, vaginally mucus, breast milk, umbilical cord blood, brain cells, spinal cord, or bone marrow.
8. The method of claim 1 , wherein the body fluid of the patient is blood.
9. The method of claim 1 , wherein said diagnosing in step (b) is also based on number of the luterial from the sample of the body fluid of the patient.
10. The method of claim 1 , wherein said diagnosing in step (b) is also based on size of the luterial from the sample of the body fluid of the patient.
11. The method of claim 1 , wherein said diagnosing in step (b) is also based on membrane formation of the luterial from the sample of the body fluid of the patient.
12. The method of claim 1 , wherein said diagnosing in step (b) is also based on nano-tracking speed of the luterial from the sample of the body fluid of the patient.
13. The method of claim 1 , wherein said diagnosing in step (b) is also based on two or more selected from the group consisting of:
number of the luterial from the sample of the body fluid of the patient;
size of the luterial from the sample of the body fluid of the patient;
membrane formation of the luterial from the sample of the body fluid of the patient; and
nano-tracking speed of the luterial from the sample of the body fluid of the patient.
14. The method of claim 1 , wherein the luterial from the sample of the body fluid of the patient prior to said imaging is stained with a fluorescent staining agent, and said imaging, by microscopy, is conducted with a fluorescent microscope.