Human serum biomarkers of prostate cancer and SARS-CoV
Anti-carbohydrate antibodies are detected by (a) contacting an array of oligomannose-serum albumin conjugates immobilized on a substrate with an antibody-containing serum sample under conditions wherein TM10 antibodies bind the oligomannose of the conjugates at at least micromolar affinity; and (b) detecting resultant binding of specific antibodies of the sample to the oligomannose of the conjugates, as indicative of the anti-carbohydrate antibodies.
1. A method of determining whether a man with a prostate specific antigen (PSA) level of <10 ng/ml has prostate cancer with a Gleason grade 4 or 5, the method comprising:
(a) contacting an array of (Man 9 GlcNAc 2 Asn)-BSA conjugates immobilized on a substrate with a blood sample from the man with the PSA level of <10 ng/ml, and detecting the presence of anti-Man 9 antibodies of the sample under conditions wherein the anti-Man 9 antibodies bind the Man 9 of the (Man 9 GlcNAc 2 Asn)-BSA conjugates, wherein Man 9 is nonamannose, GlcNac is N-acetyl-D-glucosamine, Asn is asparagine and BSA is bovine serum albumin; and
(b) measuring resultant binding of the anti-Man 9 antibodies of the sample to the Man 9 of the conjugates to determine a level of anti-Man 9 antibodies in the sample,
wherein the level of the anti-Man 9 antibodies of the sample indicates that the man with the PSA level of <10 ng/ml has prostate cancer with Gleason grade 4 or 5.
2. The method of claim 1 wherein the man has prostate hyperplasia.
3. The method of claim 1 wherein the man is post-prostatectomy.
4. The method of claim 1 wherein the array further comprises asialoorosomucoid (ASOR), galacto-orosomucoid (AGOR) or orosomucoid (OR) immobilized at distinct positions on the substrate.
5. The method of claim 1 wherein the array further comprises asialoorosomucoid (ASOR), galacto-orosomucoid (AGOR) and orosomucoid (OR) immobilized at distinct positions on the substrate.
6. The method of claim 1 , wherein the substrate is an epoxy-coated glass slide and the array is a microarray.
7. The method of claim 1 , wherein the substrate is a hydrophobic polystyrene coated plastic microplate.
8. The method of claim 1 , wherein the conjugates are immobilized by coating the surface with Man 9 -BSA at 20 μg/ml in 0.1 M Sodium Bicarbonate Buffer Solution, pH 9.6 and incubating at 37° C. for 2hrs.