Films for biologic analyte collection and analysis and methods of production and use thereof
Compositions, films, collection devices, apparatuses, kits and methods related to biologic analyte collection and analysis include thin films of modified polycaprolactone. Methods of production and use thereof are described herein. The films, compositions, collection devices, kits and methods can be used for collection of fingerprints for both image capture and nucleic acid extraction and analysis.
1. A transparent or semi-transparent hydrophilic thin film consisting essentially of polycaprolactone and a backing applied or adhered to one side of the film, the polycaprolactone having been treated with a base having a pH greater than 8 and a neutralizing agent having a thickness in the range of about 0.01 mm to about 0.6 mm that provides greater than 75% blood DNA uptake and release, wherein at least a portion of the hydrophilic film solubilizes when exposed to at least one reagent for extraction of biological analytes, and wherein the hydrophilic film has been sterilized such that it is free of nucleic acids, wherein the transparent or semi-transparent hydrophilic film is configured for collection of DNA from latent fingerprints and to obtain images of the latent fingerprints, wherein the at least one biologic analyte extraction reagent is unaffected by fingerprint visualization materials, and wherein the fingerprint visualization materials comprise ink for detecting fingerprints.
2. The transparent or semi-transparent hydrophilic thin film of claim 1 , wherein the transparent or semi-transparent hydrophilic thin film is packaged within packaging, and the packaging comprises at least one of: an identifying label imprinted with information, a radio-frequency identification tag, and a bar code.
3. The transparent or semi-transparent hydrophilic thin film of claim 1 , wherein the transparent or semi-transparent hydrophilic thin film is produced by electrospray.
4. The transparent or semi-transparent hydrophilic thin film of claim 1 , wherein the at least one reagent for extraction of biological analytes is not phenol-chloroform.
5. A transparent or semi-transparent hydrophilic thin film consisting essentially of polycaprolactone, and a backing applied or adhered to one side of the film, the film produced by:
solubilizing polycaprolactone in a solvent resulting in a solution;
applying the solution to a mold;
freezing the solution;
removing solvent from the solution resulting in a film;
subjecting the film to treatment with a base having a pH greater than 8 and a neutralizing agent under conditions that result in soluble and hydrophilic polycaprolactone; and
drying the film, wherein the film provides greater than 75% blood DNA uptake and release and has a thickness in the range of about 0.01 mm to about 0.6 mm, wherein at least a portion of the film solubilizes when exposed to at least one reagent for extraction of biological analytes, wherein the hydrophilic film has been sterilized such that it is free of nucleic acids, wherein the transparent or semi-transparent hydrophilic film is configured for collection of DNA from latent fingerprints and to obtain images of the latent fingerprints, wherein the at least one biologic analyte extraction reagent is unaffected by fingerprint visualization materials, and wherein the fingerprint visualization materials comprise ink for detecting fingerprints.
6. The transparent or semi-transparent hydrophilic thin film produced by the process of claim 5 , wherein the solution is applied to the mold using an electrospray instrument.
7. A kit for identifying a trace sample's donor, the kit comprising:
at least one transparent or semi-transparent hydrophilic thin film according to claim 1 ;
at least one biologic analyte extraction reagent excluding phenol-chloroform;
ink for detecting fingerprints, wherein the transparent or semi-transparent hydrophilic film is used for collection of DNA from latent fingerprints and to obtain images of the latent fingerprints, wherein the at least one biologic analyte extraction reagent is unaffected by fingerprint visualization materials;
packaging; and
instructions for use.
8. The kit of claim 7 , wherein the kit comprises a plurality of the transparent or semi-transparent hydrophilic films, and each transparent or semi-transparent hydrophilic thin film is individually packaged.
9. The kit of claim 8 , wherein each package comprises at least one of: an identifying label imprinted with information, a radio frequency identification tag and a bar code.
10. The kit of claim 7 , further comprising a housing having side walls coupled to a solid support and a lid, and at least one panel consisting of the transparent or semi-transparent hydrophilic thin film of claim 1 disposed in the interior of the housing.
11. The kit of claim 7 , further comprising a silica membrane spin column.