Closed system for extracting isolated medium
An assembly includes an adjustable dip tube that can selectively withdraw an isolated component at various heights within a vessel. The adjustable nature of the dip tube can improve the cell recovery rate as compared to conventional cell recovery assemblies. Additionally, the assembly can take advantage of the adjustable nature of the dip tube while maintaining an aseptic interior cavity of the assembly.
1. An assembly comprising:
a vessel having an open end, a closed end, and an interior cavity;
a cap disposed over the open end of the vessel, wherein the cap comprises a first bore extending through the cap and a second bore extending through the cap;
a tube port engaged with the first bore of the cap; and
a dynamic mechanism comprising an adjustable dip tube having a first end coupled to the cap and an opposite second end, wherein the dip tube extends a majority of the length of the interior cavity of the vessel, the adjustable dip tube adapted to selectively withdraw an isolated component in the vessel at different heights with respect to the cap, wherein the assembly is adapted to maintain the cavity as an aseptic interior cavity as the adjustable dip tube is adjusted to the different heights relative to the cap, wherein the adjustable dip tube comprises threading, wherein the tube port comprises threading,
wherein the threading on the adjustable dip tube is adapted to engage with the threading on the tube port, and wherein the tube port permits a fluid connection between the interior cavity and an exterior environment outside the adjustable dip tube.
2. The assembly of claim 1 , wherein the vessel has an open end having a maximum width WV in a range of 0.3 inches to 3 inches.
3. The assembly of claim 1 , wherein the vessel has a height HV in a range of 3 to 10 inches.
4. The assembly of claim 1 , wherein the vessel is a conical tube, a beaker, or a flask.
5. The assembly of claim 1 , wherein the dip tube includes a multimodal inner diameter.
6. The assembly of claim 5 , wherein the multimodal inner diameter is a bimodal diameter.
7. The assembly of claim 1 , wherein the first end has a first inner diameter and the opposite second end has a second inner diameter, wherein the first inner diameter is greater than the second inner diameter.
8. The assembly of claim 1 , wherein the first bore is adapted to receive the dip tube.
9. The assembly of claim 1 , wherein the second bore is adapted to receive a vent tube.
10. The assembly of claim 9 , wherein the assembly further comprises a filter and a vent tube in fluid connection with the filter.
11. The assembly of claim 1 , wherein at least a portion of the first port comprises threading, and wherein the first bore comprises threading, and wherein the threading on the first bore is adapted to engage with the threading on the first port.
12. The assembly of claim 1 , wherein the first port is disposed within the first bore and extending through the cap.
13. The assembly of claim 1 , wherein the vessel is a conical tube, a beaker, or a flask.
14. The assembly of claim 9 , wherein the vent tube is in fluid connection with a filter.
15. The assembly of claim 1 , wherein the first bore is a center bore and the second bore is an off-center bore.
16. The assembly of claim 1 , wherein the first port is disposed within the first bore and extending through the cap.
17. The assembly of claim 1 , wherein the first port comprises a tubing connector.
18. The assembly of claim 17 , wherein the tubing connector on the first port is disposed outside of the interior cavity of the vessel.
19. The assembly of claim 1 , wherein the assembly comprises a second port.
20. The assembly of claim 1 , wherein the second port is disposed within the second bore and extending through the cap.