Interference observation device and interference observation method
An interference observation apparatus includes a light source which outputs incoherent light, a beam splitter, a sample holding table, an objective lens, a reference mirror, a lens, an aberration correction plate, a piezo element, a tube lens, a beam splitter, an imaging unit, a photodetector, an image acquisition unit, and a control unit. The control unit obtains an interference intensity of combined light on the basis of a detection signal output from the photodetector, and adjusts an interference optical system to increase the interference intensity.
1. An interference observation apparatus, comprising:
a light source configured to output incoherent light;
an interference optical system configured to split the light output from the light source into first split light and second split light, reflect or transmit the first split light by an observation object, and combine the first split light and the second split light to output combined light;
a light receiving unit configured to receive the combined light and output a detection signal;
an image acquisition unit configured to acquire an interference image on the basis of the detection signal; and
a controller configured to obtain an interference intensity of the combined light on the basis of the detection signal, and adjust the interference optical system to increase the interference intensity, wherein
the light receiving unit includes an image sensor configured to capture an image of the combined light and output a first detection signal, and a photodetector configured to receive the combined light and output a second detection signal,
the image acquisition unit is configured to acquire the interference image on the basis of the first detection signal,
the controller is configured to obtain the interference intensity on the basis of the second detection signal,
the controller is configured to adjust an optical path difference between the first split light and the second split light from the splitting to the combining in the interference optical system to increase the interference intensity,
the controller is configured to obtain a phase difference in accordance with the optical path difference in the interference optical system on the basis of the second detection signal, and repeatedly perform an ON period in which the optical path difference in the interference optical system is kept constant on the basis of the phase difference and an OFF period in which the optical path difference in the interference optical system is not kept constant so that the interference optical system is adjusted while intermittently performing the ON periods, and
the image sensor is set such that a period of capturing the image of the combined light is included in the ON period.
2. The interference observation apparatus according to claim 1 , wherein the controller is configured to adjust an optical axis of an optical element on any one or both of optical paths of the first split light and the second split light in the interference optical system to increase the interference intensity.
3. The interference observation apparatus according to claim 1 , further comprising a display configured to display the interference intensity.
4. The interference observation apparatus according to claim 3 , wherein the display is configured to display a temporal variation of the interference intensity as a graph.
5. The interference observation apparatus according to claim 3 , wherein the display is configured to display the interference intensity as a numerical value.
6. The interference observation apparatus according to claim 3 , wherein the display is configured to output the interference intensity as a sound.
7. The interference observation apparatus according to claim 1 , wherein the interference optical system includes a Michelson interferometer or a Mach-Zehnder interferometer.
8. The interference observation apparatus according to claim 1 , wherein the ON period is 1 msec or more and 3 sec or less.
9. The interference observation apparatus according to claim 1 , wherein the OFF period is 30 msec or less.
10. An interference observation method, comprising:
splitting incoherent light output from a light source using an interference optical system into first split light and second split light, reflecting or transmitting the first split light by an observation object, and combining the first split light and the second split light to output combined light;
receiving the combined light using a light receiving unit and outputting a detection signal;
acquiring an interference image on the basis of the detection signal using an image acquisition unit; and
obtaining an interference intensity of the combined light on the basis of the detection signal, and adjusting the interference optical system to increase the interference intensity, wherein
the light receiving unit includes an image sensor configured to capture an image of the combined light and output a first detection signal, and a photodetector configured to receive the combined light and output a second detection signal,
the image acquisition unit is configured to acquire the interference image on the basis of the first detection signal,
the interference intensity is obtained on the basis of the second detection signal,
an optical path difference between the first split light and the second split light from the splitting to the combining in the interference optical system is adjusted to increase the interference intensity,
a phase difference in accordance with the optical path difference in the interference optical system is obtained on the basis of the second detection signal,
an ON period in which the optical path difference in the interference optical system is kept constant on the basis of the phase difference and an OFF period in which the optical path difference in the interference optical system is not kept constant are repeatedly performed so that the interference optical system is adjusted while intermittently performing the ON periods, and
a period of capturing the image of the combined light by the image sensor is set to be included in the ON period.
11. The interference observation method according to claim 10 , wherein an optical axis of an optical element on any one or both of optical paths of the first split light and the second split light in the interference optical system is adjusted to increase the interference intensity.
12. The interference observation method according to claim 10 , wherein the interference intensity is displayed using a display.
13. The interference observation method according to claim 12 , wherein the display is configured to display a temporal variation of the interference intensity as a graph.
14. The interference observation method according to claim 12 , wherein the display is configured to display the interference intensity as a numerical value.
15. The interference observation method according to claim 12 , wherein the display is configured to output the interference intensity as a sound.
16. The interference observation method according to claim 10 , wherein the interference optical system includes a Michelson interferometer or a Mach-Zehnder interferometer.
17. The interference observation method according to claim 10 , wherein the ON period is 1 msec or more and 3 sec or less.
18. The interference observation method according to claim 10 , wherein the OFF period is 30 msec or less.
19. The interference observation apparatus according to claim 1 , wherein a target phase difference in each ON period is set to the same value at every cycle.
20. The interference observation method according to claim 10 , wherein a target phase difference in each ON period is set to the same value at every cycle.