Method for manufacturing nanoparticle array, surface plasmon resonance-based sensor and method for analyzing using same
The present invention relates to a method for manufacturing a nanoparticle array, a surface plasmon resonance-based sensor, and a method for analyzing using the same. According to one embodiment of the present invention, after a mixed solution of an ionized binder and conductive nanoparticles is prepared, a substrate is dipped into the mixed solution. Thereafter, by applying an electric field to the mixed solution into which the substrate is dipped so as to induce coating of the conductive nanoparticles on the substrate, it is possible to manufacture, by a wet method, a nanoparticle array in which the conductive nanoparticles are quickly coated on the substrate with high density.
1. A method of fabricating a substrate of a sensor, the method comprising:
providing a solution comprising an ionic binder and conductive nanoparticles;
dipping the substrate into the solution; and
generating a plasma discharge over the solution having the substrate dipped therein using electrodes that are outside of the solution, thereby causing the conductive nanoparticles to be coated on the substrate.
2. The method of claim 1 , wherein the solution is in the form of a gel comprising the ionic binder and the conductive nanoparticles.
3. The method of claim 1 , wherein the ionic binder comprises one or more selected from the group consisting of poly diallyldimethylammonium chloride, poly allylamine hydrochloride, poly 4-vinylbenzyltrimethyl ammonium chloride, polyethyleneimine, poly acrylic acid, poly sodium 4-styrene sulfonate, poly vinylsulfonic acid, a poly sodium salt and a poly amino acid.
4. The method of claim 1 , wherein the substrate comprises a transparent material.
5. The method of claim 1 , wherein the substrate comprises a polymer substrate.
6. The method of claim 1 , wherein generating the plasma discharge comprises applying an electric field using the electrodes to a gas mixture comprising one or more gas(es) selected from the group consisting of helium, neon, argon, nitrogen and air.
7. The method of claim 1 , wherein the conductive nanoparticles have one or more shapes selected from the group consisting of a sphere, a tube, a column, a rod, a hollow, a pore and a wire.
8. The method of claim 1 ,
wherein the conductive nanoparticles are formed of one or more conductive materials selected from the group consisting of carbon, graphite, a metalloid, a metal, a conductive metal oxide, and a conductive metal nitride or
wherein the conductive nanoparticles comprise a dielectric core and a conductive shell, the conductive shell being formed of one or more conductive materials selected from the group consisting of carbon, graphite, a metalloid, a metal, a conductive metal oxide and a conductive metal nitride.
9. The method of claim 8 ,
wherein when the one of more conductive materials of the nanoparticles or the conductive shell includes a metalloid, the metalloid is a first element or a first alloy having an element selected from the group consisting of antimony, germanium and arsenic,
wherein when the one of more conductive materials of the nanoparticles or the conductive shell includes a metal, the metal is a second element or a second alloy having an element selected from the group consisting of titanium, zinc, aluminum, scandium, chromium, manganese, iron, cobalt, nickel, copper, indium, tin, yttrium, zirconium, niobium, molybdenum, ruthenium, rhodium, palladium, gold, silver, platinum, strontium, tungsten, cadmium and tantalum,
wherein when the one or more conductive materials of the nanoparticles or the conductive shell includes a conductive metal oxide, wherein the conductive metal oxide is formed of one or more materials selected from the group consisting of indium tin oxide, indium zinc oxide, aluminum-doped zinc oxide, gallium indium zinc oxide and zinc oxide; and
wherein when the one of more conductive materials of the nanoparticles or the conductive shell includes a conductive metal nitride, the metal nitride is formed of tungsten nitride.
10. The method of claim 1 , wherein causing the conductive nanoparticles to be coated on the substrate comprises causing the conductive nanoparticles to be arranged to propagate a surface plasmon polariton (SPP) at a wavelength corresponding to a localized surface plasmon resonance condition.
11. The method of claim 1 , further comprising attaching an immobilization molecule to at least some of the conductive nanoparticles, wherein the immobilization molecule is configured to specifically bind to one or more target analytes.
12. The method of claim 11 , wherein the immobilization molecule comprises a molecule selected from the group consisting of antigens, antibodies, proteins, peptides, DNA, RNA, PNA, enzymes, enzyme substrates, hormones, hormone receptors, and a synthetic chemical having a functional group, wherein the functional group is configured to bind to the one or more target analytes.
13. The method of claim 1 , wherein the substrate is an insulating material.
14. The method of claim 1 , wherein the substrate comprises a material selected from the group consisting of sapphire, glass, single crystal silicon, silicone rubber, latex, a metal plate, a magnetic material, a silicon oxide, and a polymer material.
15. The method of claim 14 , wherein substrate comprises the polymer material, wherein the polymer material is selected from the group consisting of polycarbonate, polyethylene terephthalate, polymethyl methacrylate, triacetyl cellulose, cyclic olefin, polyarylate, polyacrylate, polyethylene naphthelate, polybutylene terephthalate and polymide.
16. The method of claim 1 , wherein method comprises fabricating a plurality of substrates, the method comprising:
dipping a second substrate into the solution simultaneously with the substrate,
wherein the second substrate has a main surface that is separated and in parallel to a main surface of the substrate; and
wherein generating the plasma discharge causes the conductive nanoparticles to be simultaneously coated on the main surface of the substrate and the main surface of the second substrate.
17. The method of claim 1 , wherein dipping the substrate into the solution comprises submerging the substrate in the solution while generating the plasma discharge.
18. The method of claim 5 , wherein the conductive nanoparticles are caused to coat a polymeric surface of the polymer substrate.
19. The method of claim 1 , wherein generating the plasma discharge is conducted at atmospheric pressure.
20. The method of claim 1 , wherein the substrate comprises an opaque material.
21. The method of claim 1 , wherein the solution further comprises one or more solvent(s) selected from the group consisting of distilled water, deionized water, aliphatic alcohols, aliphatic ketones, eaters of aliphatic carboxylic acids, imides of aliphatic carboxylic acids, aromatic hydrocarbons, aliphatic hydrocarbons, acetonitrile and aliphatic sulfoxides.
22. The method of claim 21 , wherein the solution comprises, on the basis of a total weight of the solution, the ionic binder at 0.01 wt. %-0.3 wt. %, the conductive nanoparticles at 0.1 wt. %-0.3 wt. % and a balance comprising the solvent.