Vesicular system and uses thereof
Disclosed is a vesicular system comprising a surface with a vesicle immobilized thereon. The immobilized vesicle has a circumferential membrane of an amphiphilic polymer. The vesicle is coupled to a surface by means of a molecule with a non-polar moiety. The non-polar moiety comprises a main chain of 3 to about 30 carbon atoms and 0 to about 12 heteroatoms selected from Si, O, S, and Se. The molecule with the non-polar moiety is coupled to the surface via a covalent or non-covalent bond. A portion of the non-polar moiety is integrated in the circumferential membrane.
1. A process of forming a vesicle with an associated membrane protein, comprising:
providing a vesicle having a circumferential membrane of an amphiphilic polymer, wherein the vesicle is a synthetic polymer vesicle and the amphiphilic polymer is a synthetic block copolymer, the amphiphilic polymer comprising a molecule with a non-polar moiety, wherein a portion of the non-polar moiety of the molecule is integrated into the circumferential membrane of the vesicle, wherein the molecule with the non-polar moiety is capable of coupling the vesicle to a surface via a covalent or non-covalent bond, the non-polar moiety being one of an aliphatic, an alicyclic, an aromatic and an arylaliphatic moiety,
wherein the non-polar moiety comprises a main chain of 3 to about 30 carbon atoms and 0 to about 12 heteroatoms selected from Si, O, S, N, and Se; and
contacting the vesicle with a cell-free expression system and a nucleic acid molecule encoding the associated membrane protein under conditions suitable for transcription and translation of the nucleic acid molecule such that the associated membrane protein is formed in situ and associated with the circumferential membrane of the amphiphilic polymer of the vesicle, wherein the associated membrane protein is an odorant receptor 5 protein, an alpha-hemolysin protein, or a claudin-2 protein,
wherein a portion of the associated membrane protein is integrated into the circumferential membrane of the amphiphilic polymer of the vesicle,
wherein the amphiphilic polymer is a diblock (A-B) or a triblock copolymer (A-B-A or A-B-C),
wherein the cell-free expression system is selected from the group consisting of eukaryotic cell-free expression system, prokaryotic cell-free expression system, and archaic cell-free expression system.
2. The process of claim 1 , wherein the associated membrane protein comprises a transmembrane protein.
3. The process of claim 1 , wherein the transmembrane protein is the odorant receptor 5 protein.
4. The process of claim 1 , wherein the amphiphilic polymer has a glass transition temperature below ambient temperature.
5. The process of claim 1 , wherein the amphiphilic polymer comprises a polyether block selected from an oligo(oxyethylene) block, a poly(oxyethylene) block, an oligo(oxypropylene) block, a poly(oxypropylene) block, an oligo(oxybutylene) block and a poly(oxybutylene) block.
6. The process of claim 5 , wherein the amphiphilic polymer is a poly(butadiene)-poly(ethylene oxide) (PB-PEO) diblock copolymer.