Plant CO
The invention provides compositions and methods for manipulating the exchange of water and/or carbon dioxide (CO 2 ) through plant stomata by controlling CO 2 sensor genes. The invention provides compositions and methods for enhancing or optimizing biomass accumulation in a plant. The invention provides compositions and methods for 10 opening or closing a stomatal pore on a guard cell in the epidermis of a plant. The invention provides compositions and methods for increasing or decreasing oxygenation efficiency and/or carbon fixation in a guard cell in the epidermis of a plant by manipulating expression of a ribulose-1,5-bisphosphate carboxylase/oxygenase. The invention provides promoters for regulating expression of a nucleic acid in a plant guard cell.
1. A set of recombinant or synthetic antisense nucleic acids comprising:
(a) (i) a first nucleic acid complementary to or capable of hybridizing under stringent conditions to a sequence comprising a nucleic acid sequence encoding an amino acid sequence having at least about 95%, 96%, 97%, 98%, or 99%, or more or complete sequence identity to SEQ ID NO:3, or functional fragments thereof, wherein the first nucleic acid sequence or functional fragment nucleic acid sequence encodes a polypeptide with a β-carbonic anhydrase (carbonate dehydratase) activity, and
(ii) a second nucleic acid complementary to or capable of hybridizing under stringent conditions to a sequence comprising a nucleic acid sequence encoding an amino acid sequence having at least about 95%, 96%, 97%, 98%, or 99%, or more or complete sequence identity to SEQ ID NO:9 or functional fragments thereof, wherein the second nucleic acid or functional fragment encodes a polypeptide with a β-carbonic anhydrase (carbonate dehydratase) activity, or
(b) (i) a first nucleic acid having at least about 95%, 96%, 97%, 98%, or 99%, or more or complete sequence identity to SEQ ID NO:1 over a region of at least about 50, 75, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000, 1050, 1100, 1150 or more residues, or over the full length of a protein coding sequence (transcript) or gene, and
(ii) a second nucleic acid having at least about 95%, 96%, 97%, 98%, or 99%, or more or complete sequence identity to SEQ ID NO:7 over a region of at least about 50, 75, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000, 1050, 1100, 1150 or more residues, or over the full length of a protein coding sequence (transcript) or gene.
2. A method of inhibiting or decreasing the translation of β carbonic anhydrase protein-encoding messages in a cell or plant, or a plant or plant part, comprising administering to the cell, or a plant or plant part, or expressing in the cell, or a plant or plant part, any one of the set of recombinant or synthetic antisense nucleic acids of claim 1 .
3. A set of double-stranded inhibitory RNA (RNAi) molecules comprising:
(a) a first double stranded inhibitory RNA molecule comprising a subsequence of a recombinant or synthetic antisense nucleic acid of claim 1 (a)(i) or (b)(i), and a second double stranded inhibitory RNA molecule comprising a subsequence of a recombinant or synthetic antisense nucleic acid of claim 1 (a)(ii) or (b)(ii);
(b) the double-stranded inhibitory RNA (RNAi) molecules of (a), wherein the double-stranded inhibitory RNA are siRNA or miRNA molecules, or
(c) the double-stranded inhibitory RNA (RNAi) molecules of (a) or (b) having a length of about 20, 21, 22, 23, 24, 25 or more duplex nucleotides in length.
4. A method for up-regulating or increasing carbon dioxide (CO 2 ) and/or water exchange in a guard cell of a plant, plant cell, plant leaf, plant organ or plant part comprising;
(a) providing or having provided: the set of recombinant or synthetic nucleic acids antisense to the expression of β-carbonic anhydrase (CA) protein, gene or transcript of claim 1 ; and
(b) expressing the set of recombinant or synthetic antisense nucleic acids in the guard cell, thereby up-regulating or increasing carbon dioxide (CO 2 ) and/or water exchange in a guard cell.
5. A method for up-regulating or increasing carbon dioxide (CO 2 ) and/or water exchange in a guard cell of a plant, plant cell, plant leaf, plant organ or plant part, comprising:
(a) providing or having provided the set of double stranded inhibitory RNA molecules of claim 3 ; and
(b) expressing or having expressed the set of double stranded inhibitory RNA molecules in the guard cell, thereby up-regulating or increasing carbon dioxide (CO 2 ) and/or water exchange in a guard cell.
6. A method for regulating water exchange in a cell of a plant, plant cell, plant leaf, plant organ or plant part comprising:
expressing the set of recombinant or synthetic antisense nucleic acids of claim 1 ,
thereby under-expressing or inhibiting expression of, in the plant, plant cell, plant leaf, plant organ or plant part a polypeptide having a β-carbonic anhydrase (CA) activity, and up-regulating or increasing water exchange in the plant, plant cell, plant leaf, plant organ or plant part.
7. A method for regulating water uptake or water loss in a plant, plant cell, plant leaf, plant organ or plant part comprising under-expressing in the plant, plant cell, plant leaf, plant organ or plant part, comprising:
(A) expressing the set of double stranded RNA molecules of claim 3 ,
thereby up-regulating water exchange or increasing water loss by under-expression of the β-CA proteins in the plant, plant cell, plant leaf, plant organ or plant part;
(B) the method of (A), wherein the plant is characterized by controlled CO 2 exchange under ambient 365 ppm CO 2 , elevated ppm CO 2 or reduced ppm CO 2 , or the plant is characterized by controlled water exchange under ambient 365 ppm CO 2 , elevated ppm CO 2 or reduced ppm CO 2 ; or
(C) the method of (A) or (B), wherein the under-expression or inhibited expression is in a plant guard cell; or
(D) the method of any of (A) to (C), wherein under-expressing or inhibiting expression of a β-carbonic anhydrase protein and/or a β-carbonic anhydrase gene or transcript (message) increases water loss.
8. The set of recombinant or synthetic antisense nucleic acids of claim 1 , wherein each of the antisense oligonucleotide of (a) or (b) is between about 20 to 60, about 30 to 70, about 40 to 80, or about 60 to 100 bases in length.
9. The method of claim 4 , wherein the plant is characterized by controlled CO 2 exchange under ambient 365 ppm CO 2 , elevated ppm CO 2 or reduced ppm CO 2 .
10. The method of claim 4 , wherein the plant is characterized by controlled water exchange under ambient 365 ppm CO 2 , elevated ppm CO 2 or reduced ppm CO 2 .
11. The method of claim 6 , wherein the plant is characterized by controlled CO 2 exchange under ambient 365 ppm CO 2 , elevated ppm CO 2 or reduced ppm CO 2 , or the plant is characterized by controlled water exchange under ambient 365 ppm CO 2 , elevated ppm CO 2 or reduced ppm CO 2 .
12. The method of claim 6 , wherein the under-expressing or inhibition is in a plant guard cell.
13. The method of claim 6 , wherein under-expressing or inhibiting expression of a β-carbonic anhydrase protein and/or a β-carbonic anhydrase gene or transcript increases water exchange.
14. The method of claim 4 , wherein the plant is or is derived from:
(a) a dicotyledonous or monocotyledonous plant;
(b) wheat, oat, rye, barley, rice, sorghum , maize (corn), tobacco, a legume, a lupins, potato, sugar beet, pea, bean, soybean (soy), a cruciferous plant, a cauliflower, rape (or rapa or canola), cane (sugarcane), flax, cotton, palm, sugar beet, peanut, a tree, a poplar, a lupin, a silk cotton tree, desert willow, creosote bush, winterfat, balsa, ramie, kenaf, hemp, roselle, jute, or sisal abaca; or,
(c) a species from the genera Anacardium, Arachis, Asparagus, Atropa, Avena, Brassica, Citrus, Citrullus, Capsicum, Carthamus, Cocos, Coffea, Cucumis, Cucurbita, Daucus, Elaeis, Fragaria, Glycine, Gossypium, Helianthus, Heterocallis, Hordeum, Hyoscyamus, Lactuca, Linum, Lolium, Lupinus, Lycopersicon, Malus, Man [ iota ] hot, Majorana, Medicago, Nicotiana, Olea, Oryza, Panieum, Pannisetum, Persea, Phaseolus, Pistachia, Pisum, Pyrus, Prunus, Raphanus, Ricinus, Secale, Senecio, Sinapis, Solanum, Sorghum, Theobromus, Trigonella, Triticum, Vicia, Vitis, Vigna or Zea.
15. The method of claim 6 , wherein the plant is or is derived from:
(a) a dicotyledonous or monocotyledonous plant;
(b) wheat, oat, rye, barley, rice, sorghum , maize (corn), tobacco, a legume, a lupins, potato, sugar beet, pea, bean, soybean (soy), a cruciferous plant, a cauliflower, rape (or rapa or canola), cane (sugarcane), flax, cotton, palm, sugar beet, peanut, a tree, a poplar, a lupin, a silk cotton tree, desert willow, creosote bush, winterfat, balsa, ramie, kenaf, hemp, roselle, jute, or sisal abaca; or,
(c) a species from the genera Anacardium, Arachis, Asparagus, Atropa, Avena, Brassica, Citrus, Citrullus, Capsicum, Carthamus, Cocos, Coffea, Cucumis, Cucurbita, Daucus, Elaeis, Fragaria, Glycine, Gossypium, Helianthus, Heterocallis, Hordeum, Hyoscyamus, Lactuca, Linum, Lolium, Lupinus, Lycopersicon, Malus, Man [ iota ] hot, Majorana, Medicago, Nicotiana, Olea, Oryza, Panieum, Pannisetum, Persea, Phaseolus, Pistachia, Pisum, Pyrus, Prunus, Raphanus, Ricinus, Secale, Senecio, Sinapis, Solanum, Sorghum, Theobromus, Trigonella, Triticum, Vicia, Vitis, Vigna or Zea.
16. The method of claim 7 , wherein the plant is or is derived from:
(a) a dicotyledonous or monocotyledonous plant;
(b) wheat, oat, rye, barley, rice, sorghum , maize (corn), tobacco, a legume, a lupins, potato, sugar beet, pea, bean, soybean (soy), a cruciferous plant, a cauliflower, rape (or rapa or canola), cane (sugarcane), flax, cotton, palm, sugar beet, peanut, a tree, a poplar, a lupin, a silk cotton tree, desert willow, creosote bush, winterfat, balsa, ramie, kenaf, hemp, roselle, jute, or sisal abaca; or,
(c) a species from the genera Anacardium, Arachis, Asparagus, Atropa, Avena, Brassica, Citrus, Citrullus, Capsicum, Carthamus, Cocos, Coffea, Cucumis, Cucurbita, Daucus, Elaeis, Fragaria, Glycine, Gossypium, Helianthus, Heterocallis, Hordeum, Hyoscyamus, Lactuca, Linum, Lolium, Lupinus, Lycopersicon, Malus, Man [ iota ] hot, Majorana, Medicago, Nicotiana, Olea, Oryza, Panieum, Pannisetum, Persea, Phaseolus, Pistachia, Pisum, Pyrus, Prunus, Raphanus, Ricinus, Secale, Senecio, Sinapis, Solanum, Sorghum, Theobromus, Trigonella, Triticum, Vicia, Vitis, Vigna or Zea.
17. The method of claim 1 , wherein in step (a)(i) the amino acid has sequence as set forth in SEQ ID NO:3, or functional fragments thereof.
18. The method of claim 1 , wherein in step (a)(ii) the amino acid has a sequence as set forth in SEQ ID NO:9, or functional fragments thereof.
19. The method of claim 1 , wherein in step (b)(i) the nucleic acid has a sequence as set forth in SEQ ID NO:1, or functional fragments thereof.
20. The method of claim 1 , wherein in step (b)(ii) the nucleic acid has a sequence as set forth in SEQ ID NO:7, or functional fragments thereof.