O-acetylhomoserine sulfhydrylase variant and method for producing L-methionine using same
The present disclosure relates to a novel O-acetylhomoserine sulfhydrylase variant, a polynucleotide encoding the same, a vector comprising the polynucleotide, a strain capable of expressing the variant, and a method for producing L-methionine using the variant.
1. A modified polypeptide having an activity of O-acetylhomoserine sulfhydrylase, wherein the 196 th amino acid from the N-terminus of the polypeptide represented by the amino acid sequence of SEQ ID NO: 1, valine, is substituted with threonine.
2. A polynucleotide encoding the modified polypeptide of claim 1 .
3. A vector comprising the polynucleotide of claim 2 .
4. A microorganism of the genus Escherichia producing O-acetylhomoserine sulfhydrylase, wherein the microorganism expresses a modified polypeptide having an activity of O-acetylhomoserine sulfhydrylase, in which the 196 th amino acid from the N-terminus of the polypeptide represented by the amino acid sequence of SEQ ID NO: 1, valine, is substituted with threonine; or comprises the vector of claim 3 .
5. The microorganism of claim 4 , wherein the microorganism of the genus Escherichia is Escherichia coli.
6. A method for producing methionine, comprising reacting the modified polypeptide of claim 1 , or a microorganism producing the modified polypeptide or culture product thereof with O-acetylhomoserine and methyl mercaptan.
7. The method of claim 6 , further comprising recovering the methionine produced by the reaction.