Granted Patent
B2
US 10,383,935 · App. 15/799,465 · Granted Aug 20, 2019
Methods of making and using live attenuated viruses
Inventors:
Ryan Andrew Langlois (Minneapolis, MN); Jessica Karen Fiege (Minneapolis, MN); Louisa Elizabeth Sjaastad (Minneapolis, MN); Barbara Mae Waring (Minneapolis, MN)
Assignee:
Regents of the University of Minnesota
A61K39/145A61K39/12C12N5/0686C12N7/00C12N15/113A61K2039/5254A61K2039/575A61P31/16C12N2310/113C12N2310/141C12N2310/20C12N2501/65C12N2760/16021C12N2760/16034C12N2760/16162
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Abstract
This disclosure provides live, attenuated viruses, and methods of making and using the live, attenuated viruses.
Claims (5)
1. A cell culture of a miRNA-21 knockout cell line, the knockout cell line comprising a deletion of, or one or more mutations in, the endogenous miRNA-21 nucleic acid sequence.
2. The cell culture of claim 1 , wherein the cell line is a eukaryotic cell line.
3. The cell culture of claim 1 , wherein the cell line is a Madin-Darby Canine Kidney (MDCK) cell line.
4. The cell culture of claim 1 , wherein the miRNA-21 has the sequence shown in SEQ ID NO:13.
5. The cell culture of claim 1 , wherein the one or more mutations are selected from the group consisting of a deletion, an insertion, a point mutation, or a substitution.
Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jul 3, 2019
From: WARING, BARBARA MAE
To: REGENTS OF THE UNIVERSITY OF MINNESOTA
Reel/Frame 049662/0143 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Dec 14, 2018
From: LANGLOIS, RYAN ANDREW; FIEGE, JESSICA KAREN; SJAASTAD, LOUISA ELIZABETH
To: REGENTS OF THE UNIVERSITY OF MINNESOTA
Reel/Frame 047782/0573 →
Continuity (3)
Provisional Application
62222322
· Sep 23, 2015
Cited By (1)