Microfluidic platform and method for controlling the same
A microfluidic platform including a microfluidic layer and a contact layer. The microfluidic layer is embedded with a microfluidic structure including a micro-channel and a fluidic sample contained in the micro-channel. The contact layer is able to be attached to the microfluidic layer, and includes a first heater for heating a first area of the microfluidic structure to a first temperature and a second heater for heating a second area of the microfluidic structure to a second temperature. The microfluidic layer and the contact layer rotate together during operation. A method for controlling a sample in the micro-channel of the microfluidic structure.
1. A microfluidic platform, comprising:
a microfluidic layer comprising a micro-channel configured to receive a fluidic sample, the micro-channel emanating from a center of the microfluidic layer in a spiral curve winding around the center at a continuously increasing distance from the center, the micro-channel having a passive valve defining a constriction resulting in a decreased cross-section of the micro-channel; and
a contact layer attachable to the microfluidic layer, comprising a first heater for heating a first section of the micro-channel to a first temperature and a second heater for heating a second section of the micro-channel to a second temperature, wherein the passive valve is arranged to delineate the first section and the second section
wherein in operation, the microfluidic layer and the contact layer co-rotate to exert a force on the fluidic sample in the micro-channel, the micro-channel is configured to be filled with oil until substantially full such that the oil in the microchannel is substantially immobile as a whole, and the fluidic sample is an aqueous droplet; and
the fluidic sample is stopped by the passive valve when a rotational speed of the microfluidic layer is lower than a threshold rotational speed, and the fluidic sample is squeezed through the one of the plurality of passive valves when the rotational speed of the microfluidic layer is higher than the threshold rotational speed.
2. The microfluidic platform of claim 1 , wherein the contact layer further comprises a third heater for heating a third section of the microfluidic structure to a third temperature.
3. The microfluidic platform of claim 1 , wherein the contact layer further comprises a heat sink between the first and second heater for lowering a temperature of the fluidic sample when the fluidic sample is passing a fourth section of the microfluidic structure corresponding to the heat sink.
4. The microfluidic platform of claim 1 , further comprising:
a controller configured to control and maintain the first and second heaters to be at the first and second temperatures, respectively.
5. The microfluidic platform of claim 1 , further comprising:
a power generator coupled to and providing power to the contact layer.
6. The microfluidic platform of claim 5 , wherein the power generator is wirelessly coupled to the contact layer.
7. The microfluidic platform of claim 5 , further comprising:
a controller configured to control and maintain the first and second heaters to be at the first and second temperatures, respectively.
8. The microfluidic platform of claim 7 , wherein the controller is further configured to control the power generator to provide power to the contact layer.
9. The microfluidic platform of claim 1 , wherein a detector is arranged at the micro-channel for performing detection on the sample.
10. The microfluidic platform of claim 1 , wherein a pair of electrodes are arranged at the micro-channel for performing electrophoresis on the sample.
11. The microfluidic platform of claim 1 , wherein the heater is a resistive thin film heater, Peltier heater, or an induction heater.
12. A method for moving and heating a fluidic sample, the method comprising:
receiving a fluidic sample in a planar spiral path, the spiral path emanating from a center in a spiral curve winding around the center at a continuously increasing distance from the center, the spiral path having a first section, a second section, and a constriction between the first section and the second section;
rotating the fluidic sample at a rotational speed lower than a threshold rotational speed so that the fluidic sample is stopped by the constriction and remains in the first section;
heating the fluidic sample to a first temperature;
rotating the fluidic sample at the rotational speed higher than the threshold rotational speed so that the fluidic sample squeezes through the constriction to enter the second section; and
heating the fluidic sample to a second temperature in the second section.
13. The method of claim 12 , the method further comprising:
receiving oil to substantially full in the planar spiral path.
14. The method of claim 13 , the method further comprising:
heating the oil in the first section to the first temperature, and heating the oil in the second section to the second temperature.
15. The method of claim 12 , wherein the fluidic sample is moved and maintained repeatedly.