Influenza nucleic acid molecules and vaccines made therefrom
View Patent ↗Provided herein are nucleic acid sequences that encode novel consensus amino acid sequences of HA Influenza A of serotype H7N9 alone and in combination with HA hemagglutinin and/or influenza B hemagglutinin, as well as genetic constructs/vectors and vaccines expressing the sequences. Also provided herein are methods for generating an immune response against one or more influenza A serotypes and/or influenza B serotypes, or combinations thereof, using the vaccines that are provided.
1. An isolated nucleic acid comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO: 39, a nucleic acid sequence that is 95% identical over the entire length of the nucleic acid sequence of SEQ ID NO: 39, a fragment of SEQ ID NO: 39 comprising at least 90 nucleotides, and a nucleic acid sequence that is 95% identical to a fragment of SEQ ID NO: 39 comprising at least 90 nucleotides.
2. The isolated nucleic acid of claim 1 , wherein the isolated nucleic acid comprises a nucleic acid sequence selected from the group consisting of a nucleic acid sequence that is 95% identical over the entire length of the nucleic acid sequence of SEQ ID NO: 39 and a nucleic acid sequence that is 95% identical to a fragment of SEQ ID NO: 39 comprising at least 90 nucleotides.
3. A recombinant vector comprising the isolated nucleic acid of claim 1 .
4. An expression vector comprising the isolated nucleic acid of claim 1 operably linked to regulatory elements.
5. An expression vector comprising the isolated nucleic acid of claim 1 operably linked to regulatory elements that are functional in a human cell.
6. The expression vector of claim 5 , wherein the expression vector is a plasmid.
7. The isolated nucleic acid of claim 1 , wherein the isolated nucleic acid consists of the nucleic acid sequence of SEQ ID NO: 39.
8. A recombinant vector comprising the isolated nucleic acid of claim 7 .
9. An expression vector comprising the isolated nucleic acid of claim 7 operably linked to regulatory elements.
10. An expression vector comprising the isolated nucleic acid of claim 7 operably linked to regulatory elements that are functional in a human cell.
11. The expression vector of claim 10 , wherein the expression vector is a plasmid.
12. A composition comprising:
(a) a first nucleic acid sequence comprising one or more nucleotide sequences selected from the group consisting of SEQ ID NO: 39, a nucleic acid sequence that is 95% identical over the entire length of the nucleic acid sequence of SEQ ID NO: 39, a fragment of SEQ ID NO: 39 comprising at least 90 nucleotides, and a nucleic acid sequence that is 95% identical to a fragment of SEQ ID NO: 39 comprising at least 90 nucleotides; and
(b) a second nucleic acid sequence that encodes a protein selected from the group consisting of one or more of: H1, H2, H3, H4, H5, H6, H7, H8, H9, H10, H11, H12, H13, H14, H15, H16, N1, N2, N3, N4, N5, N6, N7, N8 and N9.
13. The composition of claim 12 , wherein the first nucleic acid sequence is operably linked to regulatory elements that are functional in a human cell.
14. The composition of claim 13 comprising a plasmid that comprises the first nucleic acid sequence operably linked to regulatory elements that are functional in a human cell.
15. The composition of claim 12 , wherein the second nucleic acid sequence is selected from the group consisting of one or more of:
SEQ ID NO: 1;
a nucleic acid sequence that is 95% identical over the entire length of the nucleic acid sequence of SEQ ID NO: 1;
a fragment of SEQ ID NO: 1 comprising at least 90 nucleotides;
a nucleic acid sequence that is 95% identical to a fragment of SEQ ID NO: 1 comprising at least 90 nucleotides;
SEQ ID NO: 3;
a nucleic acid sequence that is 95% identical over the entire length of the nucleic acid sequence of SEQ ID NO: 3;
a fragment of SEQ ID NO: 3 comprising at least 90 nucleotides;
a nucleic acid sequence that is 95% identical to a fragment of SEQ ID NO: 3 comprising at least 90 nucleotides;
SEQ ID NO: 6;
a nucleic acid sequence that is 95% identical over the entire length of the nucleic acid sequence of SEQ ID NO: 6;
a fragment of SEQ ID NO: 6 comprising at least 90 nucleotides;
a nucleic acid sequence that is 95% identical to a fragment of SEQ ID NO: 6 comprising at least 90 nucleotides;
SEQ ID NO: 8;
a nucleic acid sequence that is 95% identical over the entire length of the nucleic acid sequence of SEQ ID NO: 8;
a fragment of SEQ ID NO: 8 comprising at least 90 nucleotides; and
a nucleic acid sequence that is 95% identical to a fragment of SEQ ID NO: 8 comprising at least 90 nucleotides.
16. The composition of claim 12 , further comprising a peptide encoded by the nucleic acid sequence of SEQ ID NO: 39.
17. The composition of claim 12 , further comprising a peptide comprising the amino acid sequence of SEQ ID NO: 40.
18. A vaccine comprising the isolated nucleic acid of claim 1 .
19. The vaccine of claim 18 further comprising a peptide encoded by the nucleic acid sequence of SEQ ID NO: 39.
20. The vaccine of claim 18 further comprising a peptide comprising the amino acid sequence of SEQ ID NO: 40.
21. A vaccine comprising the nucleic acid sequence of SEQ ID NO: 39.
22. A vaccine against an influenza virus comprising a nucleic acid sequence of claim 1 .
23. The vaccine of claim 22 , wherein the nucleic acid sequence comprises the nucleic acid sequence of SEQ ID NO: 39.
24. The vaccine of claim 22 , where the influenza virus is H7N9 influenza virus.
25. The vaccine of claim 21 further comprising a peptide encoded by the nucleic acid sequence of SEQ ID NO: 39.
26. The vaccine of claim 21 further comprising a peptide comprising the amino acid sequence of SEQ ID NO: 40.