B7-related nucleic acids and polypeptides useful for immunomodulation
The present invention provides nucleic acids encoding B7-related factors that modulate the activation of immune or inflammatory response cells, such as T-cells. Also provided are expression vectors and fusion constructs comprising nucleic acids encoding B7-related polypeptides, including BSL1, BSL2, and BSL3. The present invention further provides isolated B7-related polypeptides, isolated fusion proteins comprising B7-related polypeptides, and antibodies that are specifically reactive with B7-related polypeptides, or portions thereof. In addition, the present invention provides assays utilizing B7-related nucleic acids, polypeptides, or peptides. The present invention further provides compositions of B7-related nucleic acids, polypeptides, fusion proteins, or antibodies that are useful for the immunomodulation of a human or animal subject.
1. An isolated polynucleotide consisting essentially of a sequence selected from the group consisting of:
(a) an isolated polynucleotide comprising nucleotides encoding amino acids 1 to 316 of SEQ ID NO:13;
(b) an isolated polynucleotide comprising nucleotides encoding amino acids 2 to 316 of SEQ ID NO:13; and
(c) an isolated polynucleotide comprising nucleotides encoding amino acids 29 to 316 of SEQ ID NO:13.
2. The isolated polynucleotide of claim 1 , wherein said polynucleotide is (a).
3. The isolated polynucleotide of claim 1 , wherein said polynucleotide is (b).
4. The isolated polynucleotide of claim 1 , wherein said polynucleotide is (c).
5. An isolated polynucleotide consisting essentially of the cDNA clone contained in plasmid BSL2-L165-35b in ATCC Deposit No. PTA-1988.
6. A recombinant vector comprising the isolated polynucleotide of claim 1 .
7. An isolated recombinant host cell comprising the vector of claim 6 .
8. A method of making an isolated polypeptide comprising:
(a) culturing the recombinant host cell of claim 7 under conditions such that said polypeptide is expressed; and
(b) isolating said polypeptide.
9. The isolated polynucleotide of claim 1 wherein said polynucleotide further comprises a heterologous polynucleotide.
10. The isolated polynucleotide of claim 9 wherein said heterologous polynucleotide encodes the Fc domain of human IgG.
11. An isolated polynucleotide comprising a sequence selected from the group consisting of:
(a) an isolated polynucleotide comprising nucleotides encoding amino acids 1 to 247 of SEQ ID NO:13;
(b) an isolated polynucleotide comprising nucleotides encoding amino acids 2 to 247 of SEQ ID NO:13; and
(c) an isolated polynucleotide comprising nucleotides encoding amino acids 29 to 247 of SEQ ID NO:13.
12. The isolated polynucleotide of claim 8 , wherein said polynucleotide is (a).
13. The isolated polynucleotide of claim 8 , wherein said polynucleotide is (b).
14. The isolated polynucleotide of claim 8 , wherein said polynucleotide is (c).
15. The isolated polynucleotide according to claim 12 wherein said polynucleotide comprises nucleotides 1 to 741 of SEQ ID NO:12.
16. The isolated polynucleotide according to claim 13 wherein said polynucleotide comprises nucleotides 4 to 741 of SEQ ID NO:12.
17. The isolated polynucleotide according to claim 14 wherein said polynucleotide comprises nucleotides 85 to 741 of SEQ ID NO:12.