Mogrol glycosyltransferase and gene encoding same
To provide a mogrol glycosyltransferase and a method for producing a mogrol glycoside using the enzyme. The present invention provides a mogrol glycosyltransferase and a method for producing a mogrol glycoside using the enzyme, and a transformant into which a mogrol glycosyltransferase gene is introduced and a method for preparing the transformant.
1. A method for producing a mogrol glycoside comprising reacting a protein selected from the group consisting of the following (a) to (c), a UDP-sugar, and the compound represented by formula (I) to obtain a mogrol glycoside; and purifying the mogrol glycoside:
(a) a protein consisting of the amino acid sequence of SEQ ID NO: 2;
(b) a protein consisting of an amino acid sequence in which 1 to 45 amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 2 and having an activity of adding a sugar molecule to an R 1 group at position 24 of a compound represented by the following formula (I); and
(c) a protein having an amino acid sequence having 90% or more sequence identity with the amino acid sequence of SEQ ID NO: 2 and having the activity of adding a sugar molecule to an R 1 group at position 24 of the compound represented by formula (I),
where R and R 1 each independently represent H, a monosaccharide, or a disaccharide.
2. The method according to claim 1 , wherein the UDP-sugar is a UDP-hexose.
3. The method according to claim 1 , wherein the UDP-sugar is selected from the group consisting of UDP-glucose, UDP-mannose, and UDP-galactose.
4. The method according to claim 1 , wherein the R and R 1 is each independently H or a sugar residue of a glucose monomer or a glucose dimer.
5. The method according to claim 1 , wherein the compound represented by formula (I) is mogrol or a mogrol glycoside.
6. The method according to claim 1 , which yields mogroside IA, mogroside Ib, mogroside IE, mogroside IIA, mogroside II 1 , mogroside IIE, mogroside III, mogroside IIIA 1 , mogroside IIIA 2 , mogroside IIIE, mogroside IV, mogroside IVA, mogroside V, siamenoside I, 11-oxomogroside V, mogrol pentaglycoside, or a combination thereof.
7. The method according to claim 2 , which yields mogroside IA, mogroside Ib, mogroside IE, mogroside IIA, mogroside IIA 1 , mogroside IIE, mogroside III, mogroside IIIA 1 , mogroside IIIA 2 , mogroside IIIE, mogroside IV, mogroside IVA, mogroside V, siamenoside I, 11-oxomogroside V, mogrol pentaglycoside, or a combination thereof.
8. The method according to claim 3 , which yields mogroside IA, mogroside Ib, mogroside IE, mogroside IIA, mogroside IIA 1 , mogroside IIE, mogroside III, mogroside IIIA 1 , mogroside IIIA 2 , mogroside IIIE, mogroside IV, mogroside IVA, mogroside V, siamenoside I, 11-oxomogroside V, mogrol pentaglycoside, or a combination thereof.
9. The method according to claim 4 , which yields mogroside IA, mogroside Ib, mogroside IE, mogroside IIA, mogroside IIA 1, mogroside IIE, mogroside III, mogroside IIIA 1 , mogroside IIIA 2 , mogroside IIIE, mogroside IV, mogroside IVA, mogroside V, siamenoside I, 11-oxomogroside V, mogrol pentaglycoside, or a combination thereof.
10. The method according to claim 5 , which yields mogroside IA, mogroside Ib, mogroside IE, mogroside IIA, mogroside IIA 1 , mogroside IIE, mogroside III, mogroside IIIA 1 , mogroside IIIA 2 , mogroside IIIE, mogroside IV, mogroside IVA, mogroside V, siamenoside I, 11-oxomogroside V, mogrol pentaglycoside, or a combination thereof.