Urinary crystal detection method
The present invention provides an urinary crystal detection method, which is used with a crystal collecting unit and a crystal detecting unit. The method comprises steps of using magnetic particles to attach urinary crystals from a sample. Then, providing a magnetic field to separate the urinary crystals. Further to analyz their constituent by the Raman signals of the urinary crystal to access an urinary calculus result for urinary stone patient.
1. An urinary crystal detection method, comprising steps:
providing a urine sample from a patient;
adding a plurality of magnetic particles into the urine sample, the magnetic particles attaching to a plurality of urine crystals of the urine sample, wherein the magnetic particles are formed as a core, which is coated with gelatin, and the surfaces of the magnetic particles are modified by PO(OH) 2 or PO 4 3− groups to attach the urinary crystals with Ca or Mg;
providing a magnetic field to attract the magnetic particles by a magnetic separating device, and collecting the urinary crystals which are absorbed by the magnetic particles;
analyzing a Raman signal of the urinary crystals by a microscopic Raman spectroscopy unit;
comparing the peak characteristics of the Raman signal with a component information from a database, wherein the component information is a plurality of reference peak characteristics from different urinary crystals related to a plurality of urinary calculus; and
receiving a result of the urinary calculus which the reference peak characteristics is similar as the Raman signal.
2. The urinary crystal detection method of claim 1 , wherein the component of the magnetic particles are formed by Fe 3 O 4 coated with gelatin or PSMA.
3. The urinary crystal detection method of claim 1 , wherein the size of the magnetic particle is 5 nm˜200 nm.
4. The urinary crystal detection method of claim 1 , wherein the gelatin are modified by FITC.
5. The urinary crystal detection method of claim 1 , wherein the magnetic separating device is a magnet or a magnetite.
6. The urinary crystal detection method of claim 1 , wherein the steps further comprise processing the urine sample before the step of adding the magnetic particles into the urine sample, includes:
detecting the urine sample to get a pH value; and
executing processes according to the pH value, if the pH value of urine sample is less than 7, the urine sample is executed an acid processes; if the pH value of urine sample 10 is larger than 7, the urine sample is executed an alkali processes.
7. The urinary crystal detection method of claim 6 , wherein the acid processes comprise a step of adding 5%˜15% acetic acid to the urine sample.
8. The urinary crystal detection method of claim 6 , wherein the alkali processes comprise a step of adding 5%˜15% NaOH to the urine sample.
9. The urinary crystal detection method of claim 1 , wherein the steps further comprises analyzing an auto-fluorescence signal of the urinary crystals by a fluorescence spectroscopy unit.
10. The urinary crystal detection method of claim 1 , wherein the surfaces of the magnetic particles are modified by a plurality of fluorescent molecules.
11. The urinary crystal detection method of claim 10 , wherein the fluorescent molecules are PAR or CV.
12. The urinary crystal detection method of claim 11 , wherein the steps further comprises analyzing a fluorescence signal of the fluorescent molecules by a fluorescence spectroscopy unit.