Method for modulating plant growth
The present invention provides a method for modulating non-root plant growth of a plant employing plant regulatory peptides, nucleotides encoding same, receptors therefor, binding agents thereof or agonists or antagonists thereof. Such methods encompass methods for increasing shoot growth or accelerating shoot development of plants, whereby said plants: yield a greater amount of above-ground plant matter than an untreated or wild-type plant grown under the same conditions; grows faster than an untreated or wild-type plant grown under tire same conditions; develops faster than an untreated or wild-type plant grown under the same conditions. The methods find particular application, wherein the conditions comprise stress conditions, and in particular abiotic stress conditions, which may be selected from increased salinity, drought, nitrogen limitation and pH stress.
1. A method for increasing, accelerating or increasing and accelerating non-root plant growth in a plant relative to a wild-type plant grown under the same conditions, said method comprising introducing at least one mutation or at least one exogenous nucleic acid into one or more plant cells which at least one mutation or nucleic acid results in:
(i) decreased expression of one or more C-TERMINALLY ENCODED PEPTIDEs (CEPs), decreased expression of one or more CEP receptors, or decreased expression of one or more CEPs and one or more CEP receptors by cells of a plant regenerated from or comprising said one or more plant cells, wherein said at least one mutation is in a sequence encoding a CEP, a CEP receptor or an upstream sequence thereof or wherein said exogenous nucleic acid is either a CEP-encoding sequence or a CEP receptor-encoding sequence, or said exogenous nucleic acid modulates, or its product modulates expression of at least one endogenous CEP-encoding nucleic acid or CEP receptor-encoding nucleic acid, wherein said exogenous nucleic acid comprises a nucleic acid sequence homologous to, or complementary to at least a portion of the endogenous CEP-encoding or CEP receptor-encoding nucleic acid; or
(ii) reduced affinity of one or more CEPs for their respective CEP receptors, which reduced affinity arises through modifications in the CEP(s), CEP receptor(s) or in both expressed CEP(s) and CEP receptor(s)expressed by cells of a plant regenerated from or comprising said one or more plant cells, wherein said at least one mutation is in a sequence encoding a CEP or a CEP receptor, or wherein said exogenous nucleic acid is either a CEP-encoding sequence or a CEP receptor-encoding sequence,
wherein said CEP comprises a CEP domain comprising the amino acid sequence (X 1 ) n X 2 X 3 X 4 X 5 X 6 PGX 9 SPGX 13 GX 15 (SEQ ID NO:454), wherein n is 0 or 1,
X 1 is selected from D, A, P, G, S, V, E, P, T, Q, I, N, K, and C;
X 2 is selected from F, V, T, S, A, K, Y, R, G, I, Q, H, D, G, and W;
X 3 is selected from R, A, Q, G, E, V, D, K, P, S, H, and Q;
X 4 is selected from P, G, S, T, A, N, C, H, V, E, Y, and K;
X 5 is selected from T, S, A, P, G, V, N, M, and I;
X 6 is selected from T, N, A, G, P, D, K, S, V, Y, Q, E, C, and H;
X 9 is selected from H, N, S, Y, P, R, T, G, V, F, Q, and D;
X 13 is selected from I, V, and A; and
X 15 is selected from H, and N.
2. The method of claim 1 , which comprises introducing into said one or more plant cells exogenous nucleic acid which suppresses, or the product of which suppresses expression of at least one endogenous CEP-encoding nucleic acid.
3. The method of claim 2 , wherein said exogenous nucleic acid comprises a nucleic acid sequence homologous to, or complementary to at least a portion of the endogenous CEP-encoding nucleic acid.
4. The method of claim 2 , wherein said exogenous nucleic acid is, or encodes a microRNA or siRNA.
5. The method of claim 1 , wherein said plant is selected from members of the angiosperm families Aceraceae, Anacardiaceae, Apiaceae, Asteraceae, Betulaceae, Brassicaceae, Buxaceae, Chenopodiaceae/Amaranthaceae, Compositae, Cucurbitaceae, Fabaceae, Fagaceae, Gramineae, Juglandaceae, Lamiaceae, Lauraceae, Leguminosae, Moraceae, Myrtaceae, Oleaceae, Platanaceae, Poaceae, Polygonaceae, Rosaceae, Rutaceae, Salicaceae, Solanaceae, Ulmaceae or Vitaceae or gymnosperm families Cuppressaceae, Pinaceae, Taxaceae or Taxodiaceae.
6. The method of claim 1 , wherein said CEP comprises an amino acid sequence selected from SEQ ID NOs 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36 to 147, 338-350, 365, 367, 369, 371, 373, 375, 377, 379, 381, 383, 385, 387-395, 468, 470, 472, 474, 476, 478, 480, 482, 484, 486, 488, 491, 493, 499 or 501, or comprises an CEP domain having an amino acid sequence selected from SEQ ID Nos: 148-336, 351-363, 396-415, 451-453, 455-466 or 502-504, or is encoded by a nucleic acid comprising a nucleotide sequence selected from SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 364, 366, 368, 370, 372, 374, 376, 378, 380, 382, 384, 386, 467, 469, 471, 473, 475, 477, 479, 481, 483, 485, 487, 489, 490, 492, 494-498 or 500.
7. The method of claim 6 , wherein said CEP comprises an amino acid sequence selected from SEQ ID NOs 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36 to 147, 338-350, 468, 470, 472, 474, 476, 478, 480, 482, 484, 486, 488, 491, 493, 499 or 501, or comprises a CEP domain having an amino acid sequence selected from SEQ ID Nos: 148-336, 351-363, 451, 452, 455-466 or 502-504, or is encoded by a nucleic acid comprising a nucleotide sequence selected from SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 467, 469, 471, 473, 475, 477, 479, 481, 483, 485, 487, 489, 490, 492, 494-498 or 500.
8. The method according to claim 1 , wherein said CEP comprises a CEP domain comprising an amino acid sequence (X 1 ) n X 2 X 3 X 4 X 5 X 6 PGX 9 SPGX 13 GX 15 (SEQ ID NO:454), wherein:
n may be 0 or 1
X 1 is selected from D, G, P, A, S, L, E and V;
X 2 is selected from F, V, R, T, S, A, K and Y;
X 3 is selected from R, K, E, H, Q, S, P, D, V, G, and A;
X 4 is selected from P, S and G;
X 5 is selected from T, S and G;
X 6 is selected from N, A, T, G, P, D, K and S;
X 9 is selected from N, H, Y and S;
X 13 is selected from I, A and V; and
X 15 is selected from N and H;
wherein
the amino acid at position 6 , if threonine or serine, is phosphorylated; and/or
the P at position 11, a P at position 7, a P at position 4, or any combination of such prolines are hydroxylated, or hydroxylated and arabinosylated; and/or
any combination of tyrosine residues are sulphonated.
9. The method according to claim 1 , wherein said plant yields a greater amount of above-ground plant matter than the wild-type plant grown under the same conditions.
10. The method according to claim 1 , wherein said plant grows faster than the wild-type plant grown under the same conditions.
11. The method according to claim 1 , wherein said plant develops faster than the wild-type plant grown under the same conditions.
12. The method according to claim 9 , wherein said conditions comprise stress conditions.
13. The method according to claim 12 , wherein said stress conditions are abiotic stress conditions.
14. The method according to claim 13 , wherein said stress conditions are selected from the group comprising increased salinity, drought, nitrogen limitation and pH stress.
15. A plant with increased, accelerated or increased and accelerated plant growth relative to the wild-type plant, obtained by the method of claim 1 , or a part thereof.
16. The plant of claim 15 that has increased shoot growth or accelerated shoot development relative to the wild-type plant.
17. The plant of claim 15 , wherein said plant is selected from members of the angiosperm families Aceraceae, Anacardiaceae, Apiaceae, Asteraceae, Betulaceae, Brassicaceae, Buxaceae, Chenopodiaceae/Amaranthaceae, Compositae, Cucurbitaceae, Fabaceae, Fagaceae, Gramineae, Juglandaceae, Lamiaceae, Lauraceae, Leguminosae, Moraceae, Myrtaceae, Oleaceae, Platanaceae, Poaceae, Polygonaceae, Rosaceae, Rutaceae, Salicaceae, Solanaceae, Ulmaceae or Vitaceae or gymnosperm families Cuppressaceae, Pinaceae, Taxaceae or Taxodiaceae.