Treating conditions associated with sepsis
A method for treating sepsis and/or respiratory distress syndrome (RDS) comprises applying peripheral blood from a patient or subject to an apheresis column loaded with a solid support comprising one or more binding reagents capable of specifically binding to a chemokine receptor, optionally the chemokine receptor CCR5, CXCR1, CXCR2, and/or CCR2 immobilized directly or indirectly on the support thus removing one or more chemokine receptor, optionally CCR5, CXCR1, CXCR2, and/or CCR2 expressing cells from the peripheral blood of the patient or subject. Various companion therapeutic methods and useful binding reagents are also described.
1. A method for treating sepsis in a subject in need thereof, which comprises applying peripheral blood from the subject to an apheresis column loaded with a solid support comprising one or more binding reagents capable of specifically binding to a chemokine CXCR1 receptor immobilized directly or indirectly on the support, whereby one or more cells expressing chemokine receptor CXCR1 are removed from the peripheral blood of the subject, wherein the applied blood is recirculated back into the systemic circulation of the subject, and whereby the sepsis is treated.
2. The method of claim 1 , wherein the sepsis is a systemic inflammation syndrome or causes multiple organ dysfunction syndrome.
3. The method of claim 1 , wherein the binding reagent is an agonist or an antagonist of CXCR1.
4. The method of claim 1 , wherein the binding reagent is an antibody or a chemokine.
5. The method of claim 4 , wherein the chemokine is selected from CXCL6, CXCL8.
6. The method of claim 1 , wherein the one or more cells are monocytes, macrophages, or neutrophils.
7. The method of claim 1 , wherein the subject has increased levels of expression of CXCR1 as compared to a subject that does not have sepsis.
8. The method of claim 1 , wherein 20-50% of the subject's blood is applied to the column in a single treatment.