Method of using antibody polypeptides that antagonize CD40 to treat IBD
A method of treating an inflammatory bowel disease is provided. The method comprises administration of an antibody polypeptide that specifically binds a novel epitope of human CD40. The antibody polypeptides do not exhibit CD40 agonist activity. The antibody polypeptides may be fusions of a domain antibody (dAb) comprising a single V L or V H domain and an Fc domain.
1. A method of treating an inflammatory bowel disease (IBD) in a human patient, the method comprising administering to the patient at least one dose of an antibody polypeptide comprising
(1) a variable domain Wherein the amino acid sequence of the variable domain comprises BMS3h-56-269 (SEQ ID NO: 1) or comprises (a) a CDR1 region consisting of amino acids 31 to 35 of BMS3h-56-269 as shown in SEQ ID NO: 1, (b) a CDR2 region consisting of amino acids 50 to 66 of BMS3h-56-269 as shown in SEQ ID NO: 1, and (c) a CDR3 region consisting of amino acids 99 to 105 of BMS3h-56-269 as shown in SEQ NO: 1, and
(2) a human Fe domain,
and the dose is selected from 0.5 to 300 milligrams of the antibody polypeptide for sub-cutaneous administration or from 100 to 750 mg of the antibody polypeptide for intravenous administration.
2. The method of claim 1 , wherein the administration is sub-cutaneous and the dose is selected from 0.5, 3, 10, 30, 100, 150, or 300 mg of the antibody polypeptide.
3. The method of claim 1 , wherein the administration is sub-cutaneous, the dose is from 150 to 300 mg of the antibody polypeptide, and the method comprises at least four iterations of the administering step.
4. The method of claim 1 , wherein the administration is intravenous and the dose is selected from 100 or 750 mg of the antibody polypeptide.
5. The method of claim 1 , wherein the antibody polypeptide is administered in combination with an immunosuppressive/immunomodulatory and/or anti-inflammatory agent.
6. The method of claim 5 , wherein the antibody polypeptide and the immunosuppressive/immunomodulatory and/or anti-inflammatory agent are administered sequentially.
7. The method of claim 5 , wherein the antibody polypeptide and the immunosuppressive/immunomodulatory and/or anti-inflammatory agent are administered simultaneously.
8. The method of claim 7 , Wherein the antibody polypeptide and the immunosuppressive/immunomodulatory and/or anti-inflammatory agent are formulated in a single composition.
9. The method of claim 1 , wherein the IBD is selected from the group consisting of: colitis, Crohn's disease and Behcet's disease.
10. The method of claim 9 , Wherein the colitis is selected from the group consisting of ulcerative colitis, collagenous colitis, lymphocytic colitis, and diversion colitis.
11. The method of claim 1 , wherein the Fc domain comprises an amino acid sequence selected from SEQ ID NO: 4 or SEQ ID NO: 6.
12. The method of claim 1 , wherein the antibody polypeptide further comprises a linker selected from the group consisting of: SEQ ID NOS: 8-16.
13. The method of claim 1 , wherein the amino acid sequence of the variable domain comprises BMS3h-56-269 (SEQ ID NO: 1).
14. The method of claim 13 , wherein the Fc domain (region) comprises the amino acid sequence of SEQ ID NO: 6.
15. The method of claim 1 , wherein the antibody polypeptide comprises the amino acid sequence of SEQ ID NO: 7.
16. The method of claim 1 , wherein the antibody polypeptide is linked to one or more PEG polymers.