Isolated codon optimized nucleic acid
View Patent ↗The present invention is a method for the biosynthesis of hundreds of compounds, mainly found in the Cannabis plant. The starting material for these compounds can be any biological compound that is used/produced in a biological organism from the sugar family starting materials or other low cost raw materials processed via enzymes or within organisms to give final products. These final products include, but are not limited to: cannabinoids, terpenoids, stilbenoids, flavonoids, phenolic amides, lignanamides, spermidine alkaloids, and phenylpropanoids. Specifically, the present invention relates to the regular/modified/synthetic gene(s) of select enzymes are processed and inserted into an expression system (vector, cosmid, BAC, YAC, phage, etc.) to produce modified hosts. The modified host is then optimized for efficient production and yield via manipulation, silencing, and amplifying inserted or other genes in the host, leading to an efficient system for product.
1. An isolated codon-optimized nucleic acid sequence of SEQ ID NO: 34 comprising:
a nucleotide sequence that is at least 95% identical to the nucleic acid sequence of SEQ ID NO: 34;
an isolated codon-optimized nucleic acid sequence of nucleic acids 5841 to 8867 of SEQ ID NO: 34; and
a nucleotide sequence that is at least 95% identical to the nucleic acids 5841 to 8867 of SEQ ID NO: 34;
wherein the isolated codon-optimized nucleic acid sequence of SEQ ID NO: 34 is a modified gene inserted into a host organism and thereby increasing carbon flux towards a first pathway over a second pathway in the host organism, and the modified gene is obtained by at least one of:
mutagenesis, gene knockout, overexpression, gene deletion, and promoter addition.