Flow cytometery system with fluidics control system
A system, method, and apparatus are provided for flow cytometry. In one example, the flow cytometry system includes dual laser devices and dual scatter channels to measure velocity of particles in a core stream of sample fluid. The total flow rate of the sample fluid and the sheath fluid around the sample fluid is controlled, and thus held constant, by a feedback control system controlling a vacuum pump based on differential pressure across ends of a flow channel in the flow cell.
1. A flow cytometry system, comprising:
a first scatter channel to detect a first light scatter generated by a particle passing through a first laser beam, wherein the particle flows in a flow channel of a sample fluid, wherein the first scatter channel generates first pulse information in response to detecting the first light scatter, the first pulse information including a first laser identifier and a first timestamp; and
a second scatter channel to detect a second light scatter generated by the particle flowing in the flow channel of the fluid sample and passing through a second laser beam, wherein the first laser beam and the second laser beam are separated by a first predetermined distance.
2. The flow cytometry system of claim 1 , wherein
the first scatter channel and the second scatter channel are both side scatter channels, forward scatter channels, or off angle scatter channels.
3. The flow cytometry system of claim 1 , wherein
the first scatter channel is a forward scatter channel and the second scatter channel is a side scatter channel.
4. The flow cytometry system of claim 1 , wherein
the first scatter channel is a side scatter channel and the second scatter channel is a forward scatter channel.
5. The flow cytometry system of claim 1 , further comprising:
a first laser device at a first position in a flow channel to generate the first laser beam; and
a second laser device at a second position in the flow channel to generate the second laser beam;
wherein the first laser device and the second laser device are serially separated along a center axis of the flow channel by a second predetermined distance.
6. The flow cytometry system of claim 1 , wherein
the first predetermined distance is equivalent to the second predetermined distance.
7. The flow cytometry system of claim 1 , further comprising:
a flow cell to receive a flow of the sample fluid and a flow of a sheath fluid, wherein the flow of the sample fluid is surrounded by the flow of the sheath fluid.
8. The flow cytometry system of claim 7 , further comprising:
a flow control valve to control a volumetric ratio of the sheath fluid to the sample fluid.
9. The flow cytometry system of claim 8 , wherein:
a velocity of the flow of the sample fluid is proportional to the volumetric ratio of the sheath fluid to the sample fluid.
10. The flow cytometry system of claim 8 , wherein:
the flow control valve receives feedback associated with the velocity of the sample fluid; and
the flow control valve adjusts a flow resistance of a path for the sheath fluid in response to the received feedback.
11. The flow cytometry system of claim 1 , wherein:
the second scatter channel generates second pulse information in response to detecting the second light scatter, the second pulse information including a second laser identifier and a second timestamp.
12. The flow cytometry system of claim 11 , further comprising:
an acquisition system to receive the first pulse information and the second pulse information.
13. The flow cytometry system of claim 12 , wherein:
the acquisition system calculates a laser delay for the particle by calculating a time difference between the second timestamp and the first timestamp.
14. The flow cytometry system of claim 12 , wherein:
the acquisition system includes a digital-to-analog converter (DAC) integrated in a field programmable gate array (FPGA).
15. The flow cytometry system of claim 1 , further comprising:
a pressure release valve to regulate pressure in the flow cytometry system; and
an acquisition system to control the pressure release valve.
16. The flow cytometry system of claim 15 , wherein the acquisition system:
receives a feedback signal associated with a pressure measurement in the flow cytometry system;
receives a pressure set-point and a pressure set-point tolerance; and
adjusts the pressure set-point in response to the feedback signal, the pressure set-point, and the pressure set-point tolerance.
17. The flow cytometry system of claim 1 , further comprising:
a vacuum pump to regulate vacuum pressure in the system; and
an acquisition system to control the vacuum pump.
18. The flow cytometry system of claim 1 , wherein the acquisition system:
receives a feedback signal associated with a pressure measurement in the flow cytometry system;
receives a pressure set-point, a reference laser delay, a correction constant, and a measured laser delay; and
calculates a designated pressure for the vacuum pump in response to the pressure set-point, the reference laser delay, the correction constant, and the measured laser delay.