Phase-modulation-element adjustment system and method for decreasing wavefront aberration
A phase-modulation-element adjustment system including: a positional-relationship adjustment unit that is used for a laser-scanning confocal observation apparatus including a plurality of image-forming lenses for forming a final image and at least one intermediate image and a wavefront-disturbing element and a wavefront-restoring element being placed at positions between which any of the intermediate images formed by the image-forming lenses is disposed and having opposite phase characteristics to each other and that is capable of adjusting a relative positional relationship between the wavefront-disturbing element and the wavefront-restoring element; a wavefront-aberration measurement unit for measuring a wavefront aberration of light that has come from an object and that has passed through the wavefront-disturbing element and the wavefront-restoring element; and a processor for controlling the positional-relationship adjustment unit so that the wavefront aberration measured by the wavefront-aberration measurement unit decreases.
1. A phase-modulation-element adjustment system comprising:
a positional-relationship adjustment unit that is used for a microscope apparatus including a plurality of image-forming lenses for forming a final image and at least one intermediate image and two phase-modulation elements being placed at positions between which any of the intermediate images formed by the image-forming lenses is disposed and having opposite phase characteristics to each other and that is capable of adjusting a relative positional relationship between the two phase-modulation elements;
a wavefront-aberration measurement unit for measuring a wavefront aberration of light that has come from an object and that has passed through the two phase-modulation elements; and
a control unit for controlling the positional-relationship adjustment unit so as to decrease the wavefront aberration measured by the wavefront-aberration measurement unit.
2. The phase-modulation-element adjustment system according to claim 1 , wherein the positional-relationship adjustment unit changes at least one of a position of at least one of the two phase-modulation elements in a direction along an optical axis, a position thereof in a direction intersecting the optical axis, and an angle thereof about the optical axis.
3. The phase-modulation-element adjustment system according to claim 1 , wherein the wavefront-aberration measurement unit is placed at a position farther from the object than the two phase-modulation elements are.
4. The phase-modulation-element adjustment system according to claim 1 , comprising a light source causing light from the object side to be incident on the two phase-modulation elements,
wherein the wavefront-aberration measurement unit is placed closer to the final-image side than the two phase-modulation elements are.
5. The phase-modulation-element adjustment system according to claim 4 , wherein the wavefront-aberration measurement unit includes:
a light-splitting unit for splitting the light into illumination light for observation with the microscope apparatus and reference light for adjusting the phase-modulation elements;
a multiplexing unit for multiplexing the illumination light made to pass through the two phase-modulation elements and the reference light not made to pass through the two phase-modulation elements;
an image-capturing unit for acquiring an image of interference fringes caused by an optical path difference between the illumination light and the reference light multiplexed by the multiplexing unit; and
a calculation unit for calculating the wavefront aberration on the basis of the image of interference fringes acquired by the image-capturing unit.
6. The phase-modulation-element adjustment system according to claim 1 , wherein the wavefront-aberration measurement unit is a Shack-Hartmann sensor.
7. A phase-modulation-element adjustment method of a microscope apparatus,
wherein a final image is formed by causing a first phase-modulation element to impart a spatial disturbance to a wavefront of light that comes from an object and that forms an intermediate image and by causing a second phase-modulation element to cancel out the spatial disturbance on the wavefront of the light that has formed the intermediate image, the method comprising:
a measurement step of measuring a wavefront aberration of the light that has come from the object and that has passed through the two phase-modulation elements; and
an adjustment step of adjusting a relative positional relationship between the two phase-modulation elements so as to decrease the wavefront aberration measured in the measurement step.