Virus-based biopesticide
The invention relates to synthetic combinations of two or more pure genotypes cloned from the Colombian wild-type Spodoptera frugiperda nucleopolyhedrovirus isolate (NPV003=SfCOL) and to biopesticidal compositions having an active ingredient comprising at least two synthetic combinations and, optionally, a S. frugiperda granulovirus. The compositions of the invention may contain ultraviolet protectants, diluents, coating polymers, surfactants and/or pH regulators and are effective for the biological control of insects in crops, such as corn, rice, cotton, sugarcane and grasses.
1. A biopesticidal composition comprising:
a) occlusion bodies of the nucleopolyhedrovirus NPV003, isolated from Spodoptera frugiperda further comprising the sequences sf23 and sf24, as set forth in SEQ ID NOs.: 1 and 2 respectively;
b) biological enhancer which is granulovirus VG008, characterized by a PstI restriction pattern and further characterized because its genome comprises a specific sequence SFGV24 as set forth in SEQ ID NO. 5, or proteins derived thereof; and
c) excipients selected from the group consisting of ultraviolet protectant coadjuvants, diluents, coating polymers, surfactants and/or pH regulators or combinations thereof.
2. The biopesticidal composition of claim 1 , in solid form such as powder, a granule, a tablet or a pellet, or in liquid form such as a suspension, an emulsifiable concentrate or an emulsion, optionally mixed with compost, fertilizers, bio-additives, vegetable extracts or agrochemicals.
3. The biopesticidal composition of claim 1 further comprising:
a) a concentration between 10 4 and 10 11 occlusion bodies/mL;
b) wherein the excipients are selected from diluents or carriers, pigments, coloring agents, coating polymer, potassium dihydrogen phosphate and sodium phosphate dibasic and water.
4. The biopesticidal composition of claim 1 comprising:
COMPONENT
(% w/w) on a dry basis
a concentration between
0.50-8.50
10 4 and 10 11 occlusion bodies/mL
Aluminium silicate
70.00-95.00
Red iron oxide
0.10-10.00
Lycopene-E160d
0.10-15.00
Methacrylic acid copolymer
1.00-10.00
Potassium dihydrogen phosphate
0.01-1.00
Sodium phosphate dibasic
0.10-1.00.
5. The biopesticidal composition of claim 1 comprising:
COMPONENT
(% w/w) on a dry basis
a concentration between
1.35
10 4 and 10 11 occlusion bodies/mL
Aluminium silicate
77.95
Red iron oxide
1.36
Lycopene E160d
13.61
Methacrylic acid copolymer
5.48
Potassium dihydrogen phosphate
0.04
Sodium phosphate dibasic
0.21.
6. The biopesticidal composition of claim 1 comprising:
COMPONENT
(% w/w) on a dry basis
a concentration between
2.5
10 4 and 10 11 occlusion bodies/mL
Aluminium silicate
77.8
Red iron oxide
1.36
Lycopene E160d
13.61
Methacrylic acid copolymer
4.48
Potassium dihydrogen phosphate
0.04
Sodium phosphate dibasic
0.21.
7. The biopesticidal composition of claim 1 , comprising:
COMPONENT
(% w/w) on a dry basis
a concentration between
2.5
10 4 and 10 11 occlusion bodies/mL
Aluminium silicate
77.8
Red iron oxide
2.36
Lycopene E160d
13.61
Methacrylic acid copolymer
3.48
Potassium dihydrogen phosphate
0.04
Sodium phosphate dibasic
0.21.
8. The biopesticidal composition of claim 1 , comprising:
COMPONENT
(% w/w) on a dry basis
a concentration between
0.66-6.60
10 4 and 10 11 occlusion bodies/mL
Methacrylic acid copolymer
0.00-5.00
Aluminium silicate
2.0-30.00
Lycopene E160d
0.05-5.00
Boric acid
0.05-5.00
Vegetable oil
10.00-70.00
Oleic acid
3.00-10.00
Polysorbate 80
1.00-10.00
Sorbitan Monostearate
1.0-10.00
wherein the composition is an emulsifiable concentrate.
9. The biopesticidal composition of claim 1 comprising:
COMPONENT
(% w/w) on a dry basis
a concentration between
3.4
10 8 and 10 11 occlusion bodies/mL
Methacrylic acid copolymer
0.15
Aluminium silicate
10.6
Lycopene E160d
2.84
Boric acid
0.5
Vegetable oil
66.49
Oleic acid
8.01
Polysorbate 80
4.24
Sorbitan Monostearate
3.77
wherein the composition is an emulsifiable concentrate.