Method for three-dimensional imaging using upconverting nanoparticles
The present disclosure relates to a method for three-dimensional imaging, including introducing upconverting nanoparticles into a sample, illuminating near-infrared laser such that upconverting nanoparticles introduced into a sample is excited, detecting a visible ray emitted from the excited upconverting nanoparticles and capturing and acquiring two-dimensional images by scanning the sample in a depth direction of the sample, and generating a three-dimensional image of the sample using the two-dimensional images.
1. A method for three-dimensional imaging, comprising:
introducing upconverting nanoparticles into a sample;
illuminating near-infrared laser so that the upconverting nanoparticles introduced into the sample are excited;
detecting a visible ray emitted from the excited upconverting nanoparticles while capturing and acquiring two-dimensional images by scanning the sample in a depth direction thereof; and
generating a three-dimensional image of the sample using the two-dimensional images,
wherein the generating of the three-dimensional image comprises:
obtaining coordinates on x, y and z axes according to a peak point of a light emission signal of the upconverting nanoparticles,
wherein the generating of the three-dimensional image comprises:
measuring the peak point on a Gaussian distribution curve according to an intensity of the light emission signal of the upconverting nanoparticles for the two-dimensional images on the z axis;
obtaining a coordinate on the z axis;
measuring a peak point of a two-dimensional Gaussian distribution curve for the intensity of light emission signal for an image at the obtained coordinate on the z axis; and
obtaining coordinates on the x and y axes and rendering the same, and
wherein the upconverting nanoparticles are one selected from among (NaYF 4 :Yb 3+ , Er 3+ ), (NaYF 4 :Yb 3+ , Tm 3+ ), (NaGdF 4 :Yb 3+ , Er 3+ ), (NaYF 4 :Yb 3+ , Er 3+ /NaGdF 4 ) and (NaGdF 4 :Yb 3+ , Er 3+ /NaGdF 4 ).
2. The method according to claim 1 , wherein a wavelength of the near-infrared laser is between 900 nm and 1000 nm.
3. The method according to claim 1 , wherein the near-infrared laser is 980 nm continuous wave (CW) diode laser.
4. The method according to claim 1 , wherein the acquiring of the two-dimensional images comprises:
capturing images of the sample by moving an objective lens in the depth direction of the sample.
5. The method according to claim 1 , wherein a surface of the upconverting nanoparticles are coated with a mixture of phospholipid-PEG and phospholipid PEG-amine.