Conjugates for immunotherapy
The current invention pertains to a molecular conjugate comprising an antagonist of a cell surface receptor specific to a target cell and an immune effector, such as a T cell modulator, conjugated to the antagonist. The target cell can be a cell responsible for development of a disease in a subject, for example, a cancer cell. In certain embodiments, the immune effector is an immune effector protein or an immune effector fragment thereof. The current invention also pertains to a method of treating a disease in a subject, the method comprising administering to the subject a pharmaceutically effective amount of the molecular conjugates of the current invention to the subject. The methods of the current invention can be used to treat cancer, such as breast cancer, ovarian cancer, prostate cancer, lung cancer, pancreatic cancer, or melanoma.
1. A molecular conjugate comprising:
a) a luteinizing hormone releasing hormone (LHRH) peptide antagonist; and
b) an immune effector molecule conjugated to the antagonist, wherein the immune effector molecule is selected from the group consisting of CD86, 41BBL, OX40L, or a combination of two or three thereof, or wherein the immune effector molecule is selected from the group consisting of CD40L, 41BB, CCL21, IL-12, or a combination of two or more thereof.
2. The molecular conjugate of claim 1 , wherein two or more antagonist molecules are conjugated to the immune effector molecule.
3. The molecular conjugate of claim 1 , wherein two or more immune effector molecules are conjugated to the antagonist molecule.
4. The molecular conjugate of claim 3 , wherein the two or more immune effector molecules are a single type of immune effector molecule.
5. The molecular conjugate of claim 3 , wherein the two or more immune effector molecules are two or more different types of effector molecules.
6. The molecular conjugate of claim 1 , further comprising an imaging agent.
7. The molecular conjugate of claim 1 , further comprising:
a pharmaceutically acceptable carrier.
8. A method of treating a cancer which expresses LHRH receptor, the method comprising administering to a subject in need thereof a therapeutically effective amount of a molecular conjugate according to claim 1 .
9. A method for delivering a molecular conjugate to a cancer cell expressing LHRH receptor, the method comprising administering to the cell in vitro or in vivo a molecular conjugate of claim 1 .