Particle conjugated prosthetic patches and methods of making and using thereof
Composite prosthetic patches with covalently bound particles for controlled drug release, and methods of making and using thereof, have been developed. The particles may encapsulate one or more therapeutic, prophylactic or diagnostic agent(s). Generally, the prosthetic patches are decellularized extracellular matrix such as bovine or porcine pericardium or synthetic polymeric materials. The size of the particles ranges from between 1 nm and 1000 μm, preferably from between 10 nm and 500 nm. In some embodiments, the agent is a therapeutic agent for treatment of neointimal hyperplasia. In other embodiments, the agent is a therapeutic agent for suppressing or resolving inflammation. In yet other embodiments, the agent is a therapeutic for mitigating scarring.
1. A prosthetic device comprising covalently-bound particles encapsulating one or more therapeutic, prophylactic or diagnostic agents, covalently bound within and/or to the surface of the device,
wherein the particles are covalently bound at a surface density between 0.01 and 1,000 μg/mm 2 or
wherein the particles encapsulate between 1 ng and 1 μg of the agent per 1 μg of particles,
wherein the particles release the one or more agents at a defined rate in vivo following implantation or administration of the device into a subject.
2. The prosthetic device of claim 1 , wherein the device is a prosthetic patch selected from the group consisting of a vascular patch, a cutaneous patch, a cardiac patch, and a thoracic patch.
3. The prosthetic device of claim 2 , wherein the prosthetic patch comprises natural polymers or decellularized extracellular matrix.
4. The prosthetic device of claim 1 , wherein the device is a patch selected from the group consisting of a bovine pericardium patch, a porcine pericardial patch, an autologous venous tissue patch, and a thoracic patch.
5. The prosthetic device of claim 1 wherein the particles comprise one or more biodegradable polymers.
6. The prosthetic device of claim 1 wherein the particles comprise one or more polymers selected from the group consisting of poly(lactide-co-glycolide) (PLGA), poly(lactic acid) or polylactide (PLA), poly(c-caprolactone) (PCL), poly(glycolic acid) or polyglycolide (PGA), poly(D-lactic acid) or poly(D-lactide) (PDLA), poly(L-lactic acid) or poly(L-lactide) (PLLA), polyanhydrides, and poly(ortho esters).
7. The device of claim 1 , wherein the particles are nanoparticles or microparticles with a mean diameter of between 1 nm and 1,000 μm, inclusive.
8. The device of claim 6 , wherein the particles are covalently bound at a surface density between 0.01 and 1,000 μg/mm 2 .
9. The device of claim 8 , wherein the particles encapsulate between 1 ng and 1 μg of the agent per 1 μg of particles.
10. The prosthetic device of claim 1 , wherein the particles encapsulate a therapeutic or prophylactic active agent selected from the group consisting of antiplatelet agents, anticoagulant agents, anti-inflammatory agents, antimicrobial agents, anti-metabolic agents, anti-neointima agents, immune-modulators, anti-proliferative agents, agents that affect migration and extracellular matrix production, agents that affect platelet deposition or formation of thrombus, and agents that promote vascular healing and re-endothelialization.
11. The device of claim 10 , wherein the particles encapsulate an amount of therapeutic or prophylactic active agent effective to treat or prevent one or more symptoms of a disease or disorder in the subject.
12. The device of claim 10 , wherein the total amount of the therapeutic or prophylactic active agent is between about 1 ng and 1,000 μg, inclusive.
13. The device of claim 10 , wherein the particles release the one or more agents in vivo at a constant rate following implantation or administration of the device.
14. The device of claim 10 , wherein the particles release the one or more agents in vivo over a period of between one and 31 days following implantation or administration of the device.
15. The device of claim 10 , wherein the particles release the one or more agents in vivo over a period of between one and 24 hours following implantation or administration of the device.
16. The device of claim 8 , wherein the device comprises
(a) particles that release one or more agents at a constant rate in vivo over a period of one and 24 hours; and
(b) particles that release one or more agents at a constant rate in vivo between one and 31 days following implantation or administration of the device.
17. The device of claim 1 , wherein the device has dimensions suitable for application to the subject at a location selected from the group consisting of the pericardium, a blood vessel, the brain, breast tissue, and the site of a hernial reconstruction or repair, and wherein the width, length, and height are between 0.1 mm and 300 mm, independently.
18. A method of making the device of claim 1 , the method comprising encapsulating therapeutic, prophylactic or diagnostic agent in nano or microparticles, and covalently binding an effective amount of the particles in the prosthetic wherein the agent is released from the particles in an effective amount over an effective period of time to diagnose, reduce, prevent or alleviate at least one symptom of a disease or disorder in a subject following implantation.
19. The method of claim 18 , further comprising lyophilizing the device.
20. A method of treating, preventing or diagnosing a disease or disorder in a subject, comprising implanting into a subject the device of claim 1 at a site in need thereof.
21. The device of claim 8 , wherein the particles are covalently bound at a surface density between 1 and 10 μg/mm 2 .
22. The method of claim 18 , wherein the device comprises
(a) particles that release one or more agents at a constant rate in vivo over a period of one and 24 hours; and
(b) particles that release one or more agents at a constant rate in vivo between one and 31 days following implantation or administration of the device.
23. The method of claim 18 , wherein the device is a patch selected from the group consisting of a bovine pericardium patch, a porcine pericardial patch, an autologous venous tissue patch, and a thoracic patch.
24. The method of claim 20 wherein the device has dimensions suitable for application to the subject at a location selected from the group consisting of the pericardium, a blood vessel, the brain, breast tissue, and the site of a hernial reconstruction or repair, and
wherein the width, length, and height are between 0.1 mm and 300 mm, independently.