Particle sorting apparatus and particle sorting method
The present disclosure provides a particle sorting apparatus, a particle sorting method, and a non-transitory computer-readable storage medium storing program that enable sorting object particles to be sorted with high precision, even when the sorting object particles are large. In the particle sorting apparatus, a charging unit that applies charges to at least a part of liquid droplets ejected from an orifice to generate a fluid stream and a charging control unit that adjusts a charge application end time in the charging unit according to sizes of particles included in the liquid droplets are provided.
1. A particle sorting apparatus, comprising:
a charging unit configured to apply charges to at least a portion of a plurality of liquid droplets ejected from an orifice, wherein the plurality of liquid droplets includes a plurality of particles;
a light detecting unit configured to detect an intensity of forward-scattered light generated from the plurality of particles; and
a charging control unit configured to adjust a charge application end time for application of the charges, based on a mode of the particle sorting apparatus,
wherein:
the mode is selected from one of a first mode or a second mode, based on the intensity of the forward-scattered light is less than a threshold value,
the intensity of the forward-scattered light generated from the plurality of particles included in the plurality of liquid droplets is based on a size of the plurality of particles included in the plurality of liquid droplets, and
at least one of the charge application end time or a charge application duration of the first mode is different from the second mode.
2. The particle sorting apparatus according to claim 1 , wherein the first mode is a normal mode and the second mode is a large diameter particle mode.
3. The particle sorting apparatus according to claim 1 , wherein the charging control unit is further configured to change a start time for the application of the charges, based on the size of the plurality of particles.
4. The particle sorting apparatus according to claim 1 , wherein the charging control unit is further configured to change the charge application duration based on the size of the plurality of particles.
5. The particle sorting apparatus according to claim 1 , wherein:
the orifice is in an exchangeable microchip, and
the charging unit includes a charging electrode to contact one of a sheath liquid or a sample liquid that flows through a flow channel in the exchangeable microchip.
6. The particle sorting apparatus according to claim 1 , wherein the orifice is in a flow cell.
7. A particle sorting method, comprising:
in a particle sorting apparatus:
applying charges to at least a portion of a plurality of liquid droplets ejected from an orifice; and
adjusting a charge application end time for application of the charges, based on a mode of the particle sorting apparatus,
wherein:
the mode is selected from one of a first mode or a second mode, based on an intensity of forward-scattered light is less than a threshold value,
the intensity of the forward-scattered light generated from a plurality of particles included in the plurality of liquid droplets is based on a size of the plurality of particles included in the plurality of liquid droplets, and
at least one of the charge application end time or a charge application duration of the first mode is different from the second mode.
8. A non-transitory computer-readable medium having stored thereon computer executable instructions that, when executed by a computer, cause the computer to execute operations, the operations comprising:
applying charges to at least a portion of a plurality of liquid droplets ejected from an orifice; and
adjusting a charge application end time for the application of the charges, based on a mode of a particle sorting apparatus,
wherein:
the mode is selected from one of a first mode or a second mode, based on an intensity of forward-scattered light is less than a threshold value,
the intensity of the forward-scattered light generated from a plurality of particles included in the plurality of liquid droplets is based on a size of the plurality of particles included in the plurality of liquid droplets, and
at least one of the charge application end time or a charge application duration of the first mode is different from the second mode.