Systems and devices for analysis of samples
Systems and methods for analysis of samples, and in certain embodiments, microfluidic sample analyzers configured to receive a cassette containing a sample therein to perform an analysis of the sample are described. The microfluidic sample analyzers may be used to control fluid flow, mixing, and sample analysis in a variety of microfluidic systems such as microfluidic point-of-care diagnostic platforms. Advantageously, the microfluidic sample analyzers may be, in some embodiments, inexpensive, reduced in size compared to conventional bench top systems, and simple to use. Cassettes that can operate with the sample analyzers are also described.
1. A device comprising:
a first component comprising a first channel formed in a first material and including at least a first port, the first channel including at least one portion having a cross-sectional dimension greater than 200 microns, wherein the first material has a water vapor permeability of less than about 5.0 g·mm/m 2 ·d;
a second component comprising a second channel formed in a second material and including at least a second port, the second channel including at least one portion having a cross-sectional dimension less than 200 microns, wherein the first material is different from the second material;
a fluidic connector that can be connected to the first and second components, the fluid connector comprising a fluid path including a fluid path inlet and a fluid path outlet, wherein upon connection, the fluid path inlet fluidically connects to the first port and the fluid path outlet fluidically connects to the second port to allow fluid communication between the first and second channels,
wherein the first and second channels are not in fluid communication with one another absent connection via the fluidic connector,
wherein the first and second components are substantially planar and lie on top of one another, and
wherein both the first port and the second port are accessible from a top surface of the first component or the second component.
2. A device as in claim 1 , wherein the first and second components are integrally connected to one another.
3. A device as in claim 1 , wherein the first channel of the first component is substantially enclosed by a first cover that is not the second component, and/or wherein the second channel of the second component is substantially enclosed by a second cover that is not the first component.
4. A device as in claim 3 , wherein the first and/or second cover is a tape.
5. A device as in claim 1 , wherein the first material has a water vapor permeability between about 0.01 g·mm/m 2 ·d and about 0.5 g·mm/m 2 ·d.
6. A device as in claim 1 , wherein the first material has a higher melting temperature than the second material.
7. A device as in claim 1 , wherein the second material has a melting temperature of less than about 100° C.
8. A device as in claim 1 , wherein the first and/or second material has an optical transmission of greater than 95% between 400 nm and 800 nm wavelengths of light.
9. A device as in claim 1 , wherein the first and/or second material has an optical transmission of less than 10% between 400 nm and 800 nm wavelengths of light.
10. A device as in claim 1 , wherein the first material and/or the second material comprises polystyrene, PMMA, or a cyclo-olefin copolymer.
11. A device as in claim 1 , wherein at least one portion of the first channel has a root mean square surface roughness of greater than about 5 microns, and at least one portion of the second channel has a root mean square surface roughness of less than about 5 microns.
12. A device as in claim 1 , comprising a reagent disposed in the second channel, wherein the reagent is adsorbed to a surface of at least one portion of the second channel.
13. A device as in claim 1 , wherein the second component comprises a reaction area in fluid communication with the second channel.
14. A device as in claim 1 , wherein the first channel of the first component is substantially enclosed by a first cover that is not the second component, and/or wherein the second channel of the second component is substantially enclosed by a second cover that is not the first component, wherein the first material has a water vapor permeability between about 0.01 g·mm/m 2 ·d and about 0.5 g·mm/m 2 ·d, and wherein the first material has a higher melting temperature than the second material.
15. A device as in claim 1 , wherein the first and/or second material has an optical transmission of greater than 95% between 400 nm and 800 nm wavelengths of light, and the other of the first and/or second material has an optical transmission of less than 10% between 400 nm and 800 nm wavelengths of light.
16. A device as in claim 1 , wherein the first channel of the first component is substantially enclosed by a first cover that is not the second component, and/or wherein the second channel of the second component is substantially enclosed by a second cover that is not the first component, wherein the first material has a water vapor permeability between about 0.01 g·mm/m 2 ·d and about 0.5 g·mm/m 2 ·d, wherein the first material has a higher melting temperature than the second material, and wherein the first material and/or the second material comprises polystyrene, PMMA, or a cyclo-olefin copolymer.
17. A device as in claim 1 , wherein the first channel of the first component is substantially enclosed by a first cover that is not the second component, and/or wherein the second channel of the second component is substantially enclosed by a second cover that is not the first component, wherein the first material has a water vapor permeability between about 0.01 g·mm/m 2 ·d and about 0.5 g·mm/m 2 ·d, wherein at least one portion of the first channel has a root mean square surface roughness of greater than about 5 microns, and at least one portion of the second channel has a root mean square surface roughness of less than about 5 microns.
18. A device as in claim 1 , wherein the first channel of the first component is substantially enclosed by a first cover that is not the second component, and/or wherein the second channel of the second component is substantially enclosed by a second cover that is not the first component, wherein the first material has a water vapor permeability between about 0.01 g·mm/m 2 ·d and about 0.5 g·mm/m 2 ·d, and wherein a reagent is disposed in the second channel, wherein the reagent is adsorbed to a surface of at least one portion of the second channel.
19. A device as in claim 1 , wherein the first channel of the first component is substantially enclosed by a first cover that is not the second component, and/or wherein the second channel of the second component is substantially enclosed by a second cover that is not the first component, wherein the first material has a water vapor permeability between about 0.01 g·mm/m 2 ·d and about 0.5 g·mm/m 2 ·d, and wherein the second component comprises a reaction area in fluid communication with the second channel.
20. A device as in claim 1 , wherein the first channel of the first component is substantially enclosed by a first cover that is not the second component, and/or wherein the second channel of the second component is substantially enclosed by a second cover that is not the first component, wherein the first material has a water vapor permeability between about 0.01 g·mm/m 2 ·d and about 0.5 g·mm/m 2 ·d, wherein the first material has a higher melting temperature than the second material, wherein the first material and/or the second material comprises polystyrene, PMMA, or a cyclo-olefin copolymer, and wherein a reagent is disposed in the second channel, wherein the reagent is adsorbed to a surface of at least one portion of the second channel.