Method of microbiological analysis of a sample in a unique container
The present invention relates to a method of analysis comprising the preparation and the analysis of a sample in a flexible container without direct handling of the sample and without reopening the flexible container at the end of the preparation of the sample, and also to a container and a kit that enable the implementation of this method.
1. A method of analysis of a sample comprising:
a) introducing the sample and a culture medium into a single, flexible container having a single compartment;
b) homogenizing the sample and the culture medium so as to obtain a homogenized mixture;
c) closing said container after step a) or step b);
d) enriching the homogenized mixture;
e) connecting a module to the flexible container before, during or after step d); and
f) analyzing homogenized mixture by an analysis system, wherein the module comprises the analysis system; and
wherein steps a) to d) are carried out in the single container; and steps d) to f) are carried out without reopening of the container.
2. The method of claim 1 , wherein the analysis is a microbiological analysis.
3. The method of claim 1 , wherein the flexible container comprises a homogenization zone, an opening and a socket, said socket being located outside the homogenization zone; and
the module comprises a socket adapted to be connected with the socket of the flexible container.
4. The method of claim 3 , further comprising changing the conformation of the flexible container after closure thereof and before step f) in order for the homogenized mixture to be in contact with the socket adapted to be connected with the socket of the module.
5. The method of claim 3 , wherein the socket of the flexible container is sealed when it is not connected and is adapted to allow the homogenized mixture to pass through when it is connected to the socket of the module.
6. The method of claim 1 , wherein the analysis system comprises a detector enabling the selective detection of at least one target analyte chosen from bacteria and/or proteins.
7. The method of claim 6 , wherein the detector is a specific optical or electrochemical biosensor functionalized with specific ligands.
8. The method of claim 1 , wherein the sample is solid or semi-solid.
9. The method of claim 7 , wherein said specific ligands include antibodies, aptamers and phage proteins, oligosaccharides or small organic molecules.
10. The method of claim 1 , wherein step b) comprises homogenizing mechanically by a homogenization apparatus or manually by pressing and shaking the container.