Engineered shell proteins for microcompartment shell electron transfer and catalysis
View Patent ↗Described herein are bacterial microcompartments shell proteins modified to stably incorporate iron-sulfur clusters. Such bacterial microcompartments shell proteins exhibit redox cycling and confer electron transfer functionality to bacterial microcompartment shells.
1. A bacterial microcompartment comprising a S55C-modified BMC-T1 polypeptide with a cysteine at position 55 and with at least 97% sequence identity to SEQ ID NO:3.
2. A host cell comprising the bacterial microcompartment of claim 1 .
3. The bacterial microcompartment of claim 1 , further comprising at least one BMC-H subunit.
4. The bacterial microcompartment of claim 1 , further comprising at least one BMC-P subunit.
5. The bacterial microcompartment of claim 1 , further comprising one or more BMC-T2 proteins.
6. The bacterial microcompartment of claim 1 , further comprising one or more BMC-T3 proteins.
7. The bacterial microcompartment of claim 1 , further comprising one or more iron atoms.
8. The bacterial microcompartment of claim 1 , further one or more [4Fe-4S] clusters.
9. The bacterial microcompartment of claim 1 , further comprising an enzyme.
10. The bacterial microcompartment of claim 9 , wherein the enzyme catalyzes an oxidation reaction.
11. The bacterial microcompartment of claim 1 , further comprising an enzyme that can catalyze the conversion of NAD/NADP.