Cell analysis system, cell analysis program and cell analysis method
A cell analysis system according to the present disclosure includes a motion information extracting unit and a motion characteristics calculating unit. The motion information extracting unit extracts motion information arising from a movement of ions or molecules across a cell membrane, out of a cell image obtained from imaging a cell in time series. The motion characteristics calculating unit calculates motion characteristics of the motion information.
1. A cell analysis system comprising:
an imaging apparatus configured to capture a first cell image and a second cell image; and
a non-transitory computer-readable storage medium storing a cell analysis program which operates an information processing apparatus to execute:
extracting first motion information from the first cell image and second motion information from the second cell image, wherein the first cell image and the second cell image are obtained from imaging a cell in time series;
calculating a first motion characteristic from the first motion information and a second motion characteristic from the second motion information; and
evaluating a movement of at least one ion or molecule across a cell membrane of the cell based on a difference between the first motion characteristic and the second motion characteristic.
2. The cell analysis system according to claim 1 , wherein the cell analysis program further operates the information processing apparatus to execute:
superposing the first motion characteristic and the first cell image, and generate a first motion characteristics displaying image.
3. The cell analysis system according to claim 1 , wherein the cell analysis program further operates the information processing apparatus to execute:
superposing the second motion characteristic and the second cell image, and generate a second motion characteristics displaying image.
4. The cell analysis system according to claim 1 , wherein the cell analysis program further operates the information processing apparatus to execute:
determining, based on the first motion characteristic and the second motion characteristic, one or more metrics relating to the movement of the at least one ion or molecule.
5. The cell analysis system according to claim 4 , wherein the cell analysis program further operates the information processing apparatus to execute:
calculating a motion amount, and evaluate an amount of moved ions or molecules, on the basis of the motion amount.
6. The cell analysis system according to claim 4 , wherein the cell analysis program further operates the information processing apparatus to execute:
calculating a motion amount, and evaluate an activity of an ion channel or an ionotropic receptor, on the basis of the motion amount.
7. The cell analysis system according to claim 4 , wherein the cell analysis program further operates the information processing apparatus to execute:
calculating a change in the movement of the at least one ion or molecule before and after addition of an activator or an inhibitor of an ion channel or an ionotropic receptor, and evaluate the presence or absence, or an amount, of the ion channel or the ionotropic receptor, on the basis of the change in the movement of the at least one ion or molecule.
8. The cell analysis system according to claim 4 , wherein the cell analysis program further operates the information processing apparatus to execute:
calculating a change in the movement of the at least one ion or molecule before and after addition of an inhibitor of an ionotropic receptor, and evaluate a type or a strength of a synapse formed between cells, on the basis of the change in the movement of the at least one ion or molecule.
9. The cell analysis system according to claim 4 , wherein the cell analysis program further operates the information processing apparatus to execute:
calculating a motion amount, and evaluate a type or an effectiveness of a substance that acts on an ion channel or an ionotropic receptor, on the basis of the motion amount.
10. The cell analysis system according to claim 4 , wherein the cell analysis program further operates the information processing apparatus to execute:
calculating a motion amount, and evaluate a movement, a swell, a shrink and a vibration of a cell membrane due to the movement of the at least one ion or molecule across the cell membrane or a movement of water involved, on the basis of the motion amount.
11. The cell analysis system according to claim 4 , wherein the cell analysis program further operates the information processing apparatus to execute:
calculating a motion direction, and evaluate a flow direction of the at least one ion or molecule, on the basis of the motion direction.
12. The cell analysis system according to claim 4 , wherein the cell analysis program further operates the information processing apparatus to execute:
calculating a duration time or a spatial distribution of the movement of the at least one ion or molecule, and evaluate a migration time of the at least one ion or molecule across the cell membrane or a spatial distribution of the at least one ion or molecule, on the basis of the duration time or the spatial distribution of the movement of the at least one ion or molecule.
13. The cell analysis system according to claim 1 , wherein the cell analysis program further operates the information processing apparatus to execute:
specifying an extraction range in the first cell image by using a luminance difference in the first cell image, and extract the first motion information out of the extraction range.
14. The cell analysis system according to claim 1 , wherein the cell analysis program further operates the information processing apparatus to execute:
specifying an extraction range in the second cell image by using a luminance difference in the second cell image, and extract the second motion information out of the extraction range.
15. A non-transitory computer-readable storage medium storing a cell analysis program which operates an information processing apparatus to execute:
extracting a first motion information from a first cell image and a second motion information from a second cell image, wherein the first cell image and the second cell image are obtained from imaging a cell in time series;
calculating a first motion characteristic from the first motion information and a second motion characteristic from the second motion information; and
evaluating a movement of at least one ion or molecule across a cell membrane of the cell based on a difference between the first motion characteristic and the second motion characteristic.
16. A cell analysis method comprising:
extracting a first motion information from a first cell image and a second motion information from a second cell image, wherein the first cell image and the second cell image are obtained from imaging a cell in time series;
calculating a first motion characteristic from the first motion information and a second motion characteristic from the second motion information; and
evaluating a movement of at least one ion or molecule across a cell membrane of the cell based on a difference between the first motion characteristic and the second motion characteristic.