Synthetic avian-free egg white substitute and method of making same
View Patent ↗In some embodiments, the present disclosure relates to an avian-free egg white composition including 45-63% Ovalbumin, 9-15% Ovotransferrin, 0-15% Ovomucoid, 3-5% Ovoglobulin G2, 3-5% Ovoglobulin G3, 2.5-5% Ovomucin, 3-5% Lysozyme, 1-2% Ovoinhibitor, 0.8-1.5% Ovoglycoprotein, 0.6-1.0% Flavoprotein, 0.3-0.8% Ovomacroglobulin, 0.02-0.1% Avidin, and 0.02-0.1% Cystatin. In some embodiments, the composition includes an edible yeast and one or more of the preceding proteins. In some embodiments, the avian-free egg white further includes one or more of: flavor enhancers, calcium supplements, added vitamins, and a gelling agent. In some embodiments, the present disclosure pertains to a non-allergenic egg-white composition for human consumption. In some embodiments, the present disclosure pertains to methods of making the avian-free egg-white composition.
1. An avian-free egg white substitute composition, the composition comprising:
a plurality of genetically modified micro-organisms, wherein the genetically modified micro-organisms express a plurality of different proteins selected from the group consisting of Ovalbumin, Ovotransferrin, Ovoglobulin G2, Ovoglobulin G3, Ovomucin, Lysozyme, Ovoinhibitor, Ovoglycoprotein, Flavoprotein, Ovomacroglobulin, Avidin, Ovomucoid, and Cystatin; and
wherein at least some of the plurality of different proteins remain within the genetically modified micro-organisms.
2. The composition of claim 1 , wherein the plurality of different proteins comprise Ovomucoid.
3. The composition of claim 1 , further comprising a flavor enhancer.
4. The composition of claim 3 , wherein the flavor enhancer is yeast.
5. The composition of claim 1 , further comprising a calcium supplement.
6. The composition of claim 1 , further comprising added vitamins.
7. The composition of claim 1 , further comprising a gelling agent.
8. The composition of claim 1 , further comprising algal omega-3 fatty acids.
9. The composition of claim 1 , wherein the plurality of different proteins comprise Ovalbumin, Ovotransferrin, Ovoglobulin G2, Ovoglobulin G3, Ovomucin, Lysozyme, Ovoinhibitor, Ovoglycoprotein, Flavoprotein, Ovomacroglobulin, Avidin, and Cystatin.
10. The composition of claim 9 , wherein the plurality of different proteins further comprise Ovomucoid.
11. The composition of claim 1 , wherein the genetically modified micro-organisms are in the form of edible yeast.
12. A method of making an avian-free egg white substitute, the method comprising:
expressing genes in a plurality of, genetically modified micro-organisms, wherein the genes encode a plurality of different proteins selected from the group consisting of Ovalbumin, Ovotransferrin, Ovomucoid, Ovoglobulin G2, Ovoglobulin G3, Ovomucin, Lysozyme, Ovoinhibitor, Ovoglycoprotein, Flavoprotein, Ovomacroglobulin, Avidin, Ovomucoid, and Cystatin,
wherein at least some of the plurality of different proteins remain within the genetically modified micro-organisms; and
combining the genetically modified micro-organisms to form the avian-free egg white substitute.
13. The method of claim 12 , wherein the genetically modified micro-organisms comprise multiple yeast colonies.
14. The method of claim 13 , wherein each yeast colony of the multiple yeast colonies expresses a protein selected from the group comprising: Ovalbumin, Ovotransferrin, Ovomucoid, Ovoglobulin G2, Ovoglobulin G3, Ovomucin, Lysozyme, Ovoinhibitor, Ovoglycoprotein, Flavoprotein, Ovomacroglobulin, Avidin, and Cystatin.
15. The method of claim 13 , wherein the yeast is Saccharomyces cervevisiae.
16. The method of claim 15 , wherein the yeast is Picchia pastoris.
17. The method of claim 12 , wherein the genetically modified micro-organisms are bacteria.
18. The method of claim 17 , wherein the bacteria is E. coli.
19. The method of claim 12 , wherein the avian-free egg white substitute further comprises a gelling agent.
20. The method of claim 12 , further comprising: expanding the genetically modified cells; and expressing each of the aforementioned proteins in bioreactors.
21. The composition of claim 1 , wherein the genetically modified micro-organisms comprise multiple yeast colonies.
22. The composition of claim 1 , wherein the genetically modified micro-organisms comprise bacteria.