Antibody targeting stem cell factor
Provided herein are methods, compositions, and uses relating to inhibitors of stem cell factor. For example, provided herein are antibodies targeting stem cell factor and methods for treating fibrotic and tissue remodeling diseases.
1. An isolated recombinant monoclonal anti-stem cell factor (SCF) antibody comprising:
(a) a light chain variable domain comprising an amino acid sequence comprising:
(i) a first amino acid sequence comprising the CDR1 sequence provided by amino acids 42-53 of SEQ ID NO: 4;
a second amino acid sequence comprising the CDR2 sequence provided by amino acids 69-79 of SEQ ID NO: 4; and
(iii) a third amino acid sequence comprising the CDR3 sequence provided by amino acids 112-124 of SEQ ID NO: 4; and
(b) a heavy chain variable domain comprising an amino acid sequence comprising:
(i) a first amino acid sequence comprising the CDR1 sequence provided by amino acids 45-56 of SEQ ID NO: 2;
a second amino acid sequence comprising the CDR2 sequence provided by amino acids 71-86 of SEQ ID NO: 2; and
(iii) a third amino acid sequence comprising the CDR3 sequence provided by amino acids 119-123 of SEQ ID NO: 2.
2. The antibody of claim 1 , wherein the antibody is a humanized antibody.
3. The antibody of claim 1 , wherein the antibody is a chimeric antibody.
4. The antibody of claim 1 , wherein the antibody is monovalent and comprises an Fc region.
5. The antibody of claim 1 , wherein the antibody is bivalent or bispecific.
6. The antibody of claim 1 , wherein the antibody is an antibody fragment selected from the group consisting of a Fab, a Fab′-SH, an Fv, an scFv, and a (Fab′) 2 .
7. The antibody of claim 1 , wherein the antibody comprises a single Fab region linked to an Fc region.