Oral care products and methods comprising AFPs
Provided herein are oral care compositions comprising antifreeze proteins (AFPs) useful in methods of repairing or inhibiting dental erosion, promoting dental remineralization, and/or enhancing the anti-cavity effects of fluoride.
1. A method of repairing or inhibiting or protection against or prevention of dental erosion, promoting dental remineralization, and/or enhancing the anti-cavity effects of fluoride comprising applying to the teeth an oral care composition comprising:
a) an antifreeze protein (AFP); and
b) an orally acceptable carrier,
wherein AFP has the polypeptide sequence as set forth in SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3, or a polypeptide sequence which arise from substitution, insertion or deletion of up to 5% of all amino acids, starting from the polypeptide sequence as set forth in SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3, and which still has at least 50% of the biological property of the starting protein, and wherein AFP is present in the composition in an amount of from 0.01 weight % to 3 weight % by total weight of the composition.
2. The method of claim 1 wherein the AFP is coupled to a hydroxyapatite binding peptide which is selected from the group formed from SEQ ID NO:4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, and 19.
3. The method of claim 1 wherein the AFP is a homomer or heteromer of AFPs and coupled to a hydroxyapatite binding peptide which is selected from the group formed from SEQ ID NO:4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, and 19.
4. The method described in claim 1 wherein the oral care composition further comprises an effective amount of fluoride.
5. The method of claim 1 wherein the composition is a dentifrice.
6. The method of claim 1 , wherein AFP has the polypeptide sequence as set forth in SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3, or a polypeptide sequence which arise from substitution, insertion or deletion of up to 3% of all amino acids, starting from the polypeptide sequence as set forth in SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3 and which still has at least 80% of the biological property of the starting protein, and wherein AFP is present in the composition in an amount of from 0.01 weight % to 3 weight % by total weight of the composition.
7. The method of claim 1 , wherein AFP has the polypeptide sequence as set forth in SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3, or a polypeptide sequence which arise from substitution, insertion or deletion of up to 1% of all amino acids, starting from the polypeptide sequence as set forth in SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3 and which still has at least 90% of the biological property of the starting protein, and wherein AFP is present in the composition in an amount of from 0.01 weight % to 3 weight % by total weight of the composition.
8. The method of claim 1 , wherein the AFP has the polypeptide sequence of SEQ ID NO: 1.
9. The method of claim 1 , wherein the AFP has the polypeptide sequence of SEQ ID NO: 2.
10. The method of claim 1 , wherein the AFP has the polypeptide sequence of SEQ ID NO: 3.