Methods and systems for nucleic acid analysis and quantification

1. A method for analyzing a plurality of nucleic acid molecules, comprising:

(a) providing a device comprising a plurality of partitions, wherein at least a subset of said plurality of partitions comprises said plurality of nucleic acid molecules, wherein each partition of said at least said subset of said plurality of partitions is configured to permit gas flow from said at least said subset of said plurality of partitions to an environment external to said at least said subset of said plurality of partitions through at least one thin film separating said at least said subset of said plurality of partitions from said external environment, wherein said at least one thin film is (i) substantially gas impermeable when a first pressure differential is applied across said at least one thin film and (ii) substantially gas permeable when a second pressure differential greater than said first pressure differential is applied across said at least one thin film;

(b) using a fluid flow unit to apply a pressure differential across said at least one thin film to subject gas in said plurality of partitions to flow through said at least one thin film to said external environment;

(c) while subjecting said at least said subset of said plurality of partitions to controlled heating, collecting signals from said at least said subset of said plurality of partitions; and

(d) processing said signals collected in (c) to yield data indicative of at least one melting point of at least a subset of said plurality of nucleic acid molecules in said at least said subset of said plurality of partitions.

2. The method of claim 1 , further comprising, prior to (a), performing nucleic acid amplification reactions on a nucleic acid sample under conditions sufficient to yield said plurality of nucleic acid molecules as amplification products of said nucleic acid sample.

3. The method of claim 2 , further comprising loading said nucleic acid sample into said at least said subset of said plurality of partitions prior to performing said nucleic acid amplification reactions.

4. The method of claim 2 , wherein performing nucleic acid amplification reactions on said nucleic acid sample comprises amplifying at least a portion of an internal transcribed spacer region of at least a subset of the nucleic acid molecules of said nucleic acid sample.

5. The method of claim 1 , wherein (c) comprises imaging said at least said subset of said plurality of partitions to collect said signals.

6. The method of claim 1 , wherein said processing in (d) comprises using said signals to generate signal versus temperature data for said at least said subset of said plurality of nucleic acid molecules in said at least said subset of said plurality of partitions.

7. The method of claim 1 , wherein said plurality of nucleic acid molecules are derived from a sample containing or suspected of containing a pathogen.

8. The method of claim 7 , wherein said pathogen is at least one bacterium.

9. The method of claim 8 , further comprising, prior to (a), isolating or extracting said plurality of nucleic acid molecules, or a subset thereof, from said at least one bacterium.

10. The method of claim 7 , further comprising using said data indicative of said melting point to determine a presence or absence of said pathogen in each partition of said at least said subset of said plurality of partitions.

11. The method of claim 1 , further comprising loading said plurality of nucleic acid molecules into said plurality of partitions, wherein during said loading, gas in said at least said subset of said plurality of partitions is subjected to flow from said at least said subset of said plurality of partitions through said at least one thin film to said environment external to said at least said subset of said plurality of partitions.

12. The method of claim 1 , wherein said plurality of nucleic acid molecules are derived from a blood sample.

13. The method of claim 1 , further comprising using said at least one melting point to identify one or more variants within said plurality of nucleic acid molecules.

14. The method of claim 13 , further comprising quantifying said one or more variants.

15. A method for analyzing a plurality of nucleic acid molecules, comprising:

(a) providing a device comprising a plurality of partitions, wherein at least a subset of said plurality of partitions comprises said plurality of nucleic acid molecules, wherein each partition of said at least said subset of said plurality of partitions is configured to permit gas flow from said at least said subset of said plurality of partitions to an environment external to said at least said subset of said plurality of partitions through at least one thin film separating said at least said subset of said plurality of partitions from said external environment, wherein said at least one thin film is (i) substantially gas impermeable when a first pressure differential is applied across said at least one thin film and (ii) substantially gas permeable when a second pressure differential greater than said first pressure differential is applied across said at least one thin film;

(b) using a fluid flow unit to apply a pressure differential across said at least one thin film to subject gas in said plurality of partitions to flow through said at least one thin film to said external environment;

(c) subjecting said at least said subset of said plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions using said plurality of nucleic acid molecules to generate amplification products from at least a subset of said plurality of nucleic acid molecules;

(d) while subjecting said at least said subset of said plurality of partitions to said conditions in (c), collecting signals from said at least said subset of said plurality of partitions over a plurality of time points; and

(e) processing said signals to determine a number of nucleic acid molecules in said at least said subset of said plurality of partitions.

16. The method of claim 15 , wherein subjecting said at least said subset of said plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions in (c) comprises thermal cycling, and wherein said collecting signals in (d) comprises collecting signals from each partition of said at least said subset of said plurality of partitions more than once per thermal cycle.

17. The method of claim 15 , wherein said thermal cycling is performed using a flat block thermal cycler.

18. The method of claim 15 , wherein (d) comprises imaging said at least said subset of said plurality of partitions to collect said signals.

19. The method of claim 15 , wherein (d) is performed using a detector that detects fluorescence emission at two or more wavelengths.

20. The method of claim 15 , wherein (e) comprises determining an optical intensity for each partition of said at least said subset of said plurality of partitions, wherein said optical intensity is proportional to an amount of amplification products in each of said at least said subset of said plurality of partitions.

21. The method of claim 15 , further comprising loading said plurality of nucleic acid molecules into said plurality of partitions, wherein during said loading, gas in said at least said subset of said plurality of partitions is subjected to flow from said at least said subset of said plurality of partitions through said at least one thin film to said environment external to said at least said subset of said plurality of partitions.