Processes for producing fermentation products
The present invention relates to processes for producing fermentation products from starch-containing material, wherein an alpha-amylase and optionally a thermostable protease, pullulanase and/or glucoamylase are present and/or added during liquefaction, wherein a cellulolytic composition is present and/or added during fermentation or simultaneous saccharification and fermentation. The invention also relates to a composition suitable for use in a process of the invention.
1. A process for producing ethanol from corn comprising the steps of:
i) liquefying the corn at a temperature above the initial gelatinization temperature using an alpha-amylase;
ii) saccharifying using a glucoamylase;
iii) fermenting using a fermenting organism; and
wherein a cellulolytic composition comprising an endoglucanase, a beta-glucosidase, and a cellobiohydrolase is added during saccharification or fermentation.
2. The process of claim 1 , wherein the pH during liquefaction is from 5.0-6.5.
3. The process of claim 1 , wherein the alpha-amylase is the Bacillus stearothermophilus alpha-amylase shown in SEQ ID NO: 1 or a variant thereof having at least 90% sequence identity thereto.
4. The process of claim 3 , wherein the Bacillus stearothermophilus alpha-amylase has a double deletion of positions I181+G182 and optionally a N193F substitution, or deletion of R179+G180 (using SEQ ID NO: 1 for numbering).
5. The process of claim 1 , further wherein ii) saccharifying and iii) fermentation are done simultaneously.
6. The process of claim 1 , wherein the glucoamylase is of fungal origin.
7. The process of claim 1 , wherein the cellulolytic composition is derived from a strain of Trichoderma , a strain of Humicola , or a strain of Chrysosporium.
8. The process of claim 1 , wherein the cellulolytic composition is a Trichoderma reesei cellulolytic enzyme composition further comprising Penicillium emersonii GH61A polypeptide having cellulolytic enhancing activity disclosed in SEQ ID NO: 23 herein and Aspergillus fumigatus beta-glucosidase disclosed in SEQ ID NO: 22 herein or a variant thereof with the following substitutions: F100D, S283G, N456E, F512Y.
9. The process of claim 6 , wherein the fungal origin is selected from the group consisting of a stain of Aspergillus , a strain of Trichoderma , a strain of Talaromyces , a strain of Pycnoporus , a strain of Gloeophyllum , and a strain of Nigrofomes.
10. The process of claim 6 , wherein the fungal origin is selected from the group consisting of a strain of A. niger, A. awamori, A. oryzae, T. reesei, Talaromyces emersonii, Gloeophyllum sepiarium , and Gloeophyllum trabeum.
11. The process of claim 1 , wherein the fermenting organism is a yeast.
12. The process of claim 11 , wherein the yeast is a Saccharomyces sp. yeast.
13. The process of claim 12 , wherein the yeast is a Saccharomyces cerevisiae yeast.
14. The process of claim 1 , wherein the ethanol yield is increased compared to the ethanol yield obtained without the cellulolytic composition.
15. The process of claim 14 , wherein the ethanol yield is increased by 0.73 g/L.
16. The process of claim 14 , wherein the ethanol yield is increased by up to 2.28 percent.
17. The process of claim 1 , wherein the cellobiohydrolase is cellobiohydrolase I (CBHI).
18. The process of claim 1 , wherein the cellobiohydrolase is an Aspergillus cellobiohydrolase I.
19. The process of claim 1 , wherein the cellobiohydrolase is an Aspergillus fumigatus cellobiohydrolase I.
20. The process of claim 1 , wherein the cellobiohydrolase is a Trichoderma cellobiohydrolase I.
21. The process of claim 1 , wherein the cellobiohydrolase is a Trichoderma reesei cellobiohydrolase I.
22. The process of claim 1 , wherein the cellobiohydrolase is a cellobiohydrolase II.
23. The process of claim 1 , wherein the cellobiohydrolase is an Aspergillus cellobiohydrolase II.
24. The process of claim 1 , wherein the cellobiohydrolase is an Aspergillus fumigatus cellobiohydrolase II.
25. The process of claim 1 , wherein the cellobiohydrolase is a Trichoderma cellobiohydrolase II.
26. The process of claim 1 , wherein the cellobiohydrolase is a Trichoderma reesei cellobiohydrolase II.
27. The process of claim 1 , wherein the cellobiohydrolase is a Thielavia cellobiohydrolase II.
28. The process of claim 1 , wherein the cellobiohydrolase is a Thielavia terrestris cellobiohydrolase II.