Method of forming dihydroferulic acid
The present invention relates to a method of forming dihydroferulic acid comprising providing ferulic acid and incubating the ferulic acid with a bacterial preparation of Lactobacillus johnsonii CNCM 1-1225 or a bacterial preparation of natural derivatives of Lactobacillus johnsonii CNCM 1-1225. An aspect of the invention is a composition comprising dihydroferulic acid and a bacterial preparation of Lactobacillus johnsonii CNCM 1-1225 or its natural derivatives. A further aspect of the invention is a composition comprising ferulic acid and a bacterial preparation of Lactobacillus johnsonii CNCM 1-1225 or its natural derivatives for use in the treatment or prevention of metabolic disease. Further aspects of the invention are the use of a bacterial preparation of Lactobacillus johnsonii CNCM 1-1225 or its natural derivatives; and a kit for preparing a food product.
1. A method of forming dihydroferulic acid, the method comprising providing at least one material selected from the group consisting of pure ferulic acid and hydrolyzed wheat bran; and incubating the at least one material with a bacterial preparation comprising at least one of Lactobacillus johnsonii CNCM I-1225 or a natural derivative thereof that is selected from the group consisting of L. johnsonii CNCM 1-4432, L. johnsonii CNCM 1-4433, L. johnsonii CNCM 1-4438, L. johnsonii CNCM 1-4439, L. johnsonii CNCM 1-4440, L. johnsonii CNCM 1-4442, L. johnsonii CNCM 1-4443 and combinations thereof, under conditions sufficient to produce dihydroferulic acid.
2. The method according to claim 1 wherein the bacterial preparation comprises a fermentation broth or extract of a fermentation broth of the at least one of Lactobacillus johnsonii CNCM I-1225 or natural derivative thereof.
3. The method according to claim 2 wherein the at least one of Lactobacillus johnsonii CNCM I-1225 or natural derivative thereof has been at least partially removed from the fermentation broth.
4. The method according to claim 1 wherein the at least one material is hydrolyzed wheat bran.
5. The method according to claim 4 wherein the hydrolyzed wheat bran comprises ferulic acid.
6. The method according to claim 5 wherein the bioconversion to the dihydroferulic acid is about 100% of the ferulic acid in the hydrolyzed wheat bran.
7. The method according to claim 1 wherein the at least one of Lactobacillus johnsonii CNCM I-1225 or natural derivative thereof is in a form of resting cells.
8. The method according to claim 1 wherein the at least one of Lactobacillus johnsonii CNCM I-1225 or natural derivative thereof is in a form of growing cells.
9. The method according to claim 1 wherein the incubating of the at least one material with the bacterial preparation is performed at about 37° C. for up to about 16 hours under an anaerobic condition.
10. The method according to claim 1 wherein no detectable amount of free ferulic acid is present after the incubating of the at least one material with the bacterial preparation.
11. The method according to claim 1 wherein the at least one material is pure ferulic acid.
12. The method according to claim 11 wherein the bioconversion to the dihydroferulic acid is about 100% of the pure ferulic acid.