Biotin complexes for treatment and diagnosis of Alzheimer'S disease
Described herein are compositions and methods for treating or imaging Alzheimer's disease (AD). The compositions comprise a biotin moiety and a siRNA moiety, without the need for a targeting agent such as an antibody, avidin, or streptavidin. In certain embodiments, the compositions further comprise a diagnostic agent.
1. A composition comprising a biotin moiety and a siRNA moiety, wherein the siRNA moiety comprises a nucleotide of SEQ ID NO: 2 or a nucleic acid that has at least 95% sequence homology with SEQ ID NO: 2.
2. The composition of claim 1 , wherein the composition further comprises a linker between the siRNA and the biotin.
3. The composition of claim 1 , wherein the composition does not comprise an antibody, an antigen, avidin, or streptavidin.
4. The composition of claim 1 , wherein the composition further comprises a diagnostic agent.
5. The composition of claim 4 , wherein the diagnostic agent is selected from the group consisting of a fluorophore, a chromophore, a chemoluminescing agent, a radionuclide, an unpair spin atom, a free radical, and a contrast agent.
6. The composition of claim 1 , wherein the composition is able to transfect cells in vivo.
7. The composition of claim 1 , wherein the composition partially, substantially, or completely deletes, silences, inactivates, interferes with, or down-regulates a gene that encodes selenoprotein P.
8. The composition of claim 1 , wherein the siRNA moiety comprises a guanosine at the 5′-end.
9. The composition of claim 1 , wherein the sense or the antisense strand of the siRNA moiety is equal to or less than 30 nucleotides in length.
10. A pharmaceutically acceptable formulation, wherein the pharmaceutically acceptable formulation comprises a therapeutically-effective amount of a composition of claim 1 and a pharmaceutically acceptable carrier or diluent.
11. A method generating an image of a subject, comprising the steps of:
administering to a subject in need of imaging a detectable amount of a composition of claim 4 , and
generating an image.
12. The method of claim 11 , wherein said subject is a human.