Pomegranate derived cell culture and methods for preparing and using the same

1. A scale-up process for the in vitro production of a pomegranate fruit cell culture of pomegranate aril cells grown comprising:

growing pomegranate aril cells in a flask;

inoculating the pomegranate aril cells from the flask into a first bioreactor;

inoculating the pomegranate aril cells from the first bioreactor into a second bioreactor; and

harvesting the pomegranate aril cells from the last bioreactor;

wherein the second bioreactor is a last bioreactor or an intermediate bioreactor;

wherein at least one of the first and the second bioreactor is disposable;

wherein the pomegranate aril cells harvested from the last bioreactor are dried;

wherein the size of each bioreactor used in the process is larger than the one in which the pomegranate aril cells were previously grown;

wherein the pomegranate aril cells harvested from the last bioreactor comprise a total polyphenol concentration of between 100,000-700,000 mg/kg dry weight powder;

wherein the pomegranate aril cells are grown in a growth medium comprising Gamborg B5 medium at pH of 5.4-5.8 comprising 2-4% sucrose, 0.2-0.3 g/L casein hydrolysate, 0.05-0.15 g/L myo inositol, 0.05-0.15 mg/L 1-naphthaleneacetic acid (NAA) and, 0.1-0.3 mg/L kinetin, 25-45 mM KNOB, 1-15 mM MgSO 4 or 5-35 mM MgNO 3 and 1 mM NaH 2 PO 4 .

2. The process of claim 1 , wherein if the second bioreactor is an intermediate bioreactor, an additional step of inoculating the pomegranate aril cells to another bioreactor is performed.

3. The process of claim 1 , wherein any one of the bioreactor is a 4-10 liter bioreactor.

4. The process of claim 1 , wherein any one of the bioreactor is a 10-50 liter bioreactor.

5. The process of claim 1 , wherein any one of the bioreactor is a 50-200 liter bioreactor.

6. The process of claim 1 , wherein any one of the bioreactor is a 200-500 liter bioreactor.

7. The process of claim 1 , any one of the bioreactor is a 200-1000 liter bioreactor.

8. The process of claim 1 , wherein the disposable bioreactor is made from one or more layers of polyethylene.

9. The process of claim 8 , wherein the disposable bioreactor is made from an inner and outer layer of polyethylene and a middle nylon layer.

10. The process of claim 1 , wherein the growth medium includes plant hormones.

11. A composition in a form of a powder produced by the method of claim 1 and having a total polyphenol concentration of between 100,000-700,000 mg/kg dry weight powder.

12. The composition according to claim 11 , wherein the pomegranate aril cells include a complex of polyphenols, punicalagin, 1,2,3,4,6-pentagalloyl glucose (PGG) or a combination thereof.

13. A method of treating inflammation comprising:

administering an effective amount of the composition of claim 11 to a subject in need.