Molecular signatures of invasive cancer subpopulations
Carcinomas typically invade as a cohesive multicellular unit, a process termed collective invasion. It remains unclear how different subpopulations of cancer cells contribute to this process. We developed three-dimensional (3D) organoid assays to identify the most invasive cancer cells in primary breast tumors. Collective invasion was led by specialized cancer cells that were defined by their expression of basal epithelial genes, such as cytokeratin-14 (K14) and p63. Furthermore, K14+ cells led collective invasion in the major human breast cancer subtypes. Importantly, lumenal cancer cells were observed to convert phenotypically to invasive leaders following induction of basal epithelial genes. Although only a minority of cells within lumenal tumors expressed basal epithelial genes, knockdown of either K14 or p63 was sufficient to block collective invasion. Our data reveal that heterotypic interactions between epithelial subpopulations are critical to collective invasion. We suggest that targeting the basal invasive program could limit metastatic progression.
1. A method comprising the steps of:
a. isolating a primary tumors from a subject;
b. generating tumor organoids via mechanical disruption and enzymatic digestion;
c. separating tumor organoids from single cells using differential centrifugation;
d. embedding the tumor organoids in an extracellular matrix gel; and
e. measuring gene expression and/or protein levels in the cells leading the invasive strands of the tumor organoids.
2. The method of claim 1 , wherein the extracellular matrix gel is a collagen I gel.
3. The method of claim 1 , wherein the extracellular matrix gel is a three-dimensional extracellular matrix gel.
4. A method comprising the steps of:
a. isolating tissue from a patient's tumor;
b. generating tumor organoids via mechanical disruption and enzymatic digestion of the tissue;
c. separating tumor organoids from single cells using differential centrifugation;
d. embedding the tumor organoids in an extracellular matrix gel; and
e. performing an invasion assay on the tumor organoids.
5. The method of claim 4 , wherein the extracellular matrix gel is a collagen I gel.
6. The method of claim 4 , wherein the extracellular matrix gel is a three-dimensional extracellular matrix gel.
7. The method of claim 4 , further comprising the step of measuring gene expression and/or protein levels in the cells leading the invasive strands of the tumor organoids.