Observation apparatus
An observation device includes: a structured illumination section; a phase difference measurement illumination section; a phase contrast lens that has a phase plate for dimming illumination light for phase difference measurement, where the illumination light for phase difference measurement is incident into the lens, and the structured illumination light is incident into the lens from a side opposite to an incidence side of the ring-shaped illumination; a detection section that detects reflected light of the structured illumination light; and an observation section that images the illumination light for phase difference measurement. When Fourier transform is performed on the structured illumination light, a spatial frequency of the structured illumination light is set on a high-frequency side or a low-frequency side with respect to a position of the phase plate on optical Fourier space.
1. An observation apparatus comprising:
a structured illumination grid and light source that emits structured illumination light having a light-dark pattern;
a phase difference measurement illumination slit and objective lens that emits illumination light for phase difference measurement onto a subject-mount surface;
a phase contrast lens that has a phase plate for dimming illumination light for phase difference measurement, where the illumination light for phase difference measurement having passed through the subject-mount surface is incident into the phase contrast lens, and the structured illumination light is incident into the phase contrast lens in a direction different from a direction of incidence of the illumination light for phase difference measurement through an optical system;
a sensor that detects reflected light of the structured illumination light which is reflected on the subject-mount surface; and
a set of an objective lens and sensor that images the illumination light for phase difference measurement which has passed through the phase contrast lens,
wherein a spatial frequency of the structured illumination light is set such that a position of an image of the structured illumination light applied Fourier transform is positioned on a high-frequency side or a low-frequency side with respect to a position of the phase plate on optical Fourier space, by adjusting a pattern of the grid.
2. The observation apparatus according to claim 1 , further comprising a focus position adjustment section that adjusts a focus position of the phase contrast lens, on the basis of an intensity of the reflected light detected by the sensor.
3. The observation apparatus according to claim 1 , wherein a plurality of the phase contrast lenses having different magnifications is configured to be interchangeable, and
wherein the spatial frequency of the structured illumination light is changed in accordance with interchange of the phase contrast lenses.
4. The observation apparatus according to claim 2 , wherein a plurality of the phase contrast lenses having different magnifications is configured to be interchangeable, and
wherein the spatial frequency of the structured illumination light is changed in accordance with interchange of the phase contrast lenses.
5. The observation apparatus according to claim 3 ,
wherein a spatial frequency of the grid is changed in accordance with interchange of the phase contrast lenses.
6. The observation apparatus according to claim 3 ,
wherein a wavelength of the light emitted from light source is changed in accordance with interchange of the phase contrast lenses.
7. The observation apparatus according to claim 3 , wherein a magnification of the optical system is changed in accordance with interchange of the phase contrast lenses.
8. The observation apparatus according to claim 1 , wherein the set of the objective lens and sensor comprises an imaging element that captures an image of the illumination light for phase difference measurement having passed through the subject-mount surface and the phase contrast lenses.
9. The observation apparatus according to claim 2 , wherein the set of the objective lens and sensor comprises an imaging element that captures an image of the illumination light for phase difference measurement having passed through the subject-mount surface and the phase contrast lenses.
10. The observation apparatus according to claim 3 , wherein the set of the objective lens and sensor comprises an imaging element that captures an image of the illumination light for phase difference measurement having passed through the subject-mount surface and the phase contrast lenses.
11. The observation apparatus according to claim 5 , wherein the set of the objective lens and sensor comprises an imaging element that captures an image of the illumination light for phase difference measurement having passed through the subject-mount surface and the phase contrast lenses.
12. The observation apparatus according to claim 6 , wherein the set of the objective lens and sensor comprises an imaging element that captures an image of the illumination light for phase difference measurement having passed through the subject-mount surface and the phase contrast lenses.
13. The observation apparatus according to claim 1 , wherein the illumination light for phase difference measurement is ring-shaped illumination light.
14. The observation apparatus according to claim 1 , wherein the structured illumination light is incident into the phase contrast lenses in a direction opposite to a direction of incidence of the illumination light for phase difference measurement.