System and method for transferring tissue
A transfer device designed to extract an amorphous or semi-solid structure, tissue, or construct from supporting media while maintaining the spatial integrity/organizational architecture thereof. The transfer device can include a controller, an actuator assembly, a plunger, and a needle. The controller can move the transfer device and the plunger independently.
1. A method for transferring a tissue structure using a transfer device, the transfer device including a controller operably coupled with the transfer device and a plunger, the plunger operably coupled with a needle, the method comprising:
depressing, by a pre-selected first amount and according to instructions by the controller, the plunger towards the needle;
moving the transfer device, according to instructions by the controller, towards a first location of the at least two transfer locations;
inserting the needle into the first medium to a pre-selected depth and maintaining the first pre-selected distance between the plunger and the first medium, according to instructions by the controller, the first medium being located at the first location, the first medium including the tissue structure, the inserted needle surrounding the tissue structure and a sacrificial amount of the first medium;
removing the needle, the plunger, the tissue structure, and the sacrificial amount while maintaining the first pre-selected distance between the plunger and the sacrificial amount, according to instructions from the controller, a second pre-selected distance from the first medium;
moving the transfer device, according to instructions from the controller, to a second location of the at least two transfer locations;
inserting the needle, the tissue structure, and the sacrificial amount into the second medium and maintaining the first pre-selected distance between the plunger and the sacrificial amount, according to instructions from the controller, to a second pre-selected depth in a second medium; and
withdrawing the needle at a first rate and depressing the plunger at a second rate based at least on the first rate, according to instructions from the controller, the combination of the withdrawing and the depressing depositing the tissue structure and the sacrificial amount in the second medium.
2. The method as in claim 1 further comprising:
printing the tissue structure into the first medium.
3. The method as in claim 1 wherein the first medium comprises a carbomer.
4. The method as in claim 1 wherein the second medium comprises a carbomer.
5. The method as in claim 1 wherein at least one of the at least two transfer locations comprises a multiple well plate.
6. The method as in claim 1 wherein at least one of the at least two transfer locations comprises a bioreactor.
7. A transfer system for transferring a tissue structure from a first location of at least two locations to a second location of the at least two locations, the transfer system comprising:
a transfer device including a needle and a plunger operably coupled with the needle;
a controller operably coupled with the transfer device and the plunger, the controller executing instructions, the instructions including:
depressing, by a pre-selected first amount, the plunger towards the needle;
moving the transfer device towards the first location;
inserting the needle into the first medium to a pre-selected depth and maintaining the first pre-selected distance between the plunger and the first medium, the first medium being located at the first location, the first medium including the tissue structure, the inserted needle surrounding the tissue structure and a sacrificial amount of the first medium;
removing, by a second pre-selected distance from the first medium, the needle, the plunger, the tissue structure, and the sacrificial amount while maintaining the first pre-selected distance between the plunger and the sacrificial amount;
moving the transfer device to the second location;
inserting, to a second pre-selected depth in a second medium, the needle, the tissue structure, and the sacrificial amount and maintaining the first pre-selected distance between the plunger and the sacrificial amount; and
withdrawing the needle at a first rate and depressing the plunger at a second rate based at least on the first rate, the combination of the withdrawing and the depressing leaving the tissue structure and the sacrificial amount in the second medium.
8. The system as in claim 7 wherein the instructions further comprise:
printing the tissue structure into the first medium.
9. The system as in claim 7 wherein the first medium comprises a carbomer.
10. The system as in claim 7 wherein the second medium comprises a carbomer.
11. The system as in claim 7 wherein at least one of the at least two transfer locations comprises a multiple well plate.
12. The system as in claim 7 wherein at least one of the at least two transfer locations comprises a bioreactor.