Methods and compositions for enhancing the efficacy and specificity of RNA silencing
The present invention provides methods of enhancing the efficacy and specificity of RNA silencing. The invention also provides compositions for mediating RNA silencing. In particular, the invention provides siRNAs, siRNA-like molecules, shRNAs, vectors and transgenes having improved specificity and efficacy in mediating silencing of a target gene. Therapeutic methods are also featured.
1. A method of silencing a target mammalian mRNA in a cell, comprising contacting said cell with an engineered RNA precursor, wherein the engineered RNA precursor comprises a duplex stem with a first stem portion and second stem portion that is complementary to the first stem portion and a loop connecting the first and second stem portions, wherein said engineered RNA precursor is derived from a pre-miRNA and wherein, upon contacting, the engineered RNA precursor is processed to a double stranded RNA (dsRNA), said dsRNA comprising a sense and an antisense strand, in which said sense and antisense strand each possess a 5′ and a 3′ end, wherein said antisense strand is complementary to the target mammalian mRNA and directs cleavage by a RISC complex at a phosphodiester bond within the target mRNA, and wherein the base pair strength between the 5′ end of the antisense strand (AS 5′) and the 3′ end of the sense strand (S 3′) is less than the base pair strength between the 3′ end of the antisense strand (AS 3′) and the 5′ end of the sense strand (S 5′), due to the introduction of a modification in the duplex stem of the engineered RNA precursor corresponding to the AS 5′ and the S 3′ end of the dsRNA,
wherein the engineered RNA precursor comprises the modification relative to an initial engineered RNA precursor that is processed to produce a dsRNA that directs RISC cleavage at the same phosphodiester bond within the target,
wherein the modification is selected from the group consisting of:
(a) at least one mismatched base pair;
(b) at least one wobble base pair;
(c) at least one base pair comprising a rare nucleotide; and
(d) at least one base pair comprising a modified amino-purine nucleotide.
2. The method of claim 1 , wherein said dsRNA comprises at least one mismatched dTdT tail comprising two unpaired deoxythymidine (dT) nucleotides.
3. The method of claim 1 , wherein said dsRNA comprises two mismatched dTdT tails comprising two unpaired deoxythymidine (dT) nucleotides on the AS 3′ and the S 3′.
4. The method of claim 1 , wherein said target mRNA comprises superoxide dismutase 1 (SOD1) mRNA.
5. The method of claim 4 , wherein said sense strand comprises a sequence selected from the group consisting of SEQ ID NOs: 13, 15, 75, 84, 85 and 86 and wherein said antisense strand comprises a sequence selected from the group consisting of SEQ ID NOs: 14, 16, 20, 76 and 77.
6. The method of claim 4 , wherein said SOD1 mRNA comprises a sequence selected from the group consisting of SEQ ID NOs: 11 and 73.
7. The method of claim 6 , wherein said sense strand comprises a sequence selected from the group consisting of SEQ ID NOs: 13, 15, 75, 84, 85 and 86 and wherein said antisense strand comprises a sequence selected from the group consisting of SEQ ID NOs: 14, 16, 20, 76 and 77.
8. The method of claim 7 , wherein said engineered RNA precursor is encoded by a transgene.
9. The method of claim 8 , wherein said transgene is carried by a viral vector.
10. The method of claim 9 , wherein said viral vector comprises an adeno-associated virus.
11. A method of silencing a target mammalian mRNA in a cell, comprising contacting said cell with an engineered RNA precursor, wherein the engineered RNA precursor comprises a duplex stem with a first stem portion and second stem portion that is complementary to the first stem portion and a loop connecting the first and second stem portions, wherein said engineered RNA precursor is derived from a pre-miRNA and comprises a duplex stem comprising the nucleotide sequences of a siRNA duplex desired to be produced in vivo, and wherein, upon contacting the cell, the engineered RNA precursor is processed to produce the siRNA duplex, said siRNA duplex comprising a sense and an antisense strand, in which said sense and antisense strand each possess a 5′ and a 3′ end, wherein said antisense strand is complementary to the target mammalian mRNA and directs cleavage by a RISC complex at a phosphodiester bond within the target mRNA, and wherein the base pair strength between the 5′ end of the antisense strand (AS 5′) and the 3′ end of the sense strand (S 3′) is less than the base pair strength between the 3′ end of the antisense strand (AS 3′) and the 5′ end of the sense strand (S 5′), due to the introduction of a modification in the duplex stem of the engineered RNA precursor corresponding to the AS 5′ and the S 3′ end of the siRNA duplex,
wherein the engineered RNA precursor comprises the modification relative to an initial engineered RNA precursor that is processed to produce a siRNA duplex that directs RISC cleavage at the same phosphodiester bond within the target mRNA, wherein the modification is selected from the group consisting of:
(a) at least one mismatched base pair;
(b) at least one wobble base pair;
(c) at least one base pair comprising a rare nucleotide; and
(d) at least one base pair comprising a modified amino-purine nucleotide.
12. The method of claim 11 , wherein said engineered RNA precursor is encoded by a transgene carried by a viral vector.
13. The method of claim 12 , wherein said viral vector comprises an adeno-associated virus.
14. The method of claim 1 , wherein the engineered RNA precursor is chemically synthesized.
15. The method of claim 11 , wherein the engineered RNA precursor is chemically synthesized.
16. The method of claim 1 , wherein the AS 5′ and the S 3′ of said dsRNA comprise a mismatched base pair selected from the group consisting of G:A, C:A, C:U, G:G, A:A, C:C, U:U, and U:T.
17. The method of claim 1 , wherein the AS 5′ and the S 3′ of said dsRNA comprise a wobble base pair selected from the group consisting of G:U and G:T.
18. The method of claim 1 , wherein the AS 5′ and the S 3′ of said dsRNA comprise an inosine (I) rare nucleotide.
19. The method of claim 18 , wherein the AS 5′ and the S 3′ of said dsRNA comprise a base pair comprising a rare nucleotide selected from the group consisting of I:A, I:U and I:C.
20. The method of claim 1 , wherein the modified nucleotide is an amino-purine nucleotide selected from the group consisting of 2-amino-G, 2-amino-A, 2,6-diamino-G, and 2,6-diamino-A.
21. The method of claim 11 , wherein the AS 5′ and the S 3′ of said siRNA duplex comprise a mismatched base pair selected from the group consisting of G:A, C:A, C:U, G:G, A:A, C:C, U:U, and U:T.
22. The method of claim 11 , wherein the AS 5′ and the S 3′ of said siRNA duplex comprise a wobble base pair selected from the group consisting of G:U and G:T.
23. The method of claim 11 , wherein the AS 5′ and the S 3′ of said siRNA duplex comprise an inosine (I) rare nucleotide.
24. The method of claim 23 , wherein the AS 5′ and the S 3′ of said siRNA duplex comprise a base pair comprising a rare nucleotide selected from the group consisting of I:A, I:U and I:C.
25. The method of claim 11 , wherein the modified nucleotide is an amino-purine nucleotide selected from the group consisting of 2-amino-G, 2-amino-A, 2,6-diamino-G, and 2,6-diamino-A.