IP Library Granted Patent US 10,619,198
Granted Patent B2
US 10,619,198 · App. 15/545,996 · Granted Apr 14, 2020

PCR primer linked to complementary nucleotide sequence or complementary nucleotide sequence including mis-matched nucleotides and method for amplifying nucleic acid using the same

Inventors: Yoo-Deok Won (Yongin-si, KR); Hae-joon Park (Yongin-si, KR); Sunyoung Lee (Yongin-sl, KR)
Assignee: SD BIOSENSOR, Inc.
C12Q1/6853C12Q1/68C12Q1/686
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 10,619,198
App. No.
15/545,996
Granted
Apr 14, 2020
Kind
B2
Abstract

The present invention relates to a primer for PCR obtained by, directly or through inosine as a linker, linking a complementary nucleotide sequence or a complementary nucleotide sequence including a mis-matched nucleotide sequence to the 5′-terminal of a forward or reverse primer; and to a PCR method including a step of mixing a nucleic acid template in a PCR composition including the primer and then performing PCR on the mixture. The primer for PCR of the present invention includes a complementary nucleotide sequence or a mis-matched nucleotide sequence in a complementary nucleotide sequence, which is linked to the 5′-terminal thereof directly or via a linker, thereby lowering the sensitivity increase due to the increase in amplification products and reducing non-specifically occurring reactions in PCR.

Claims (2)

1. A primer for gene amplification, wherein the primer is selected from the group consisting of SEQ ID NOs: 4 to 15.

2. A kit comprising the primer of claim 1 .

Priority Claims (1)
KR 10-2015-0015276 · Jan 30, 2015 · national
Continuity (1)
Related Publication 20180002746A1 · Jan 4, 2018
Cited By (1)
US 12,698,525