Methods and compositions for stimulating immune response using potent immunostimulatory RNA motifs
The presently disclosed subject matter relates to methods and compositions relating to immunostimulatory RNA motifs that act as adjuvants and/or immunostimulatory agents to enhance host immune responses.
1. An isolated RNA molecule comprising the nucleotide sequence set forth in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:5, or SEQ ID NO:23.
2. The isolated RNA molecule of claim 1 comprising the nucleotide sequence set forth in SEQ ID NO: 1, wherein 5-15 nucleotides are added to the 5′ end of the nucleotide sequence.
3. The isolated RNA molecule of claim 2 , wherein 5-15 nucleotides are added to the 3′ end of the nucleotide sequence.
4. A pharmaceutical composition comprising:
the isolated RNA molecule of claim 1 ; and
a pharmaceutically acceptable carrier.
5. The pharmaceutical composition of claim 4 , further comprising an adjuvant.
6. The pharmaceutical composition of claim 4 further comprising one or more antigens.
7. The pharmaceutical composition of claim 6 , wherein the one or more antigens are selected from the group consisting of virus, bacterial, fungal, parasite, nucleotide, polysaccharides, lipids, tumor antigens, allergens, and peptide antigens.
8. The isolated RNA molecule of claim 1 , wherein the isolated RNA molecule has immunostimulatory activity.