Method and apparatus for diagnosing a tumor using a histogram and excluding abnormal fluorescence corresponding to DNA aneuploidy
An apparatus and a method for diagnosing a tumor are provided. A histogram generating section generates a histogram from a result of a measurement of fluorescence intensity of a suspension produced from a target sample. The histogram indicates a relation between the fluorescence intensity and the number of cells. A determination section detects an S phase fluorescence intensity range corresponding to S phase cells based on the fluorescence intensity corresponding to a peak of the number of cells in the histogram, detects an abnormal fluorescence intensity range corresponding to tumor cells, based on a change in the number of cells in the S phase fluorescence intensity range, and performs a diagnosis of a tumor in the target sample based on an index of the number of cells in a range defined by excluding the abnormal fluorescence intensity range from the S phase fluorescence intensity range.
1. A tumor diagnostic apparatus comprising:
a non-transitory computer readable medium storing a program;
and a processor configured to execute the program including the following the steps:
generating a histogram from a result of a measurement of fluorescence intensity of a suspension, the suspension having been produced from a target sample and comprising a cell containing DNA that is fluorescently stained, the histogram being indicative of a relation between the fluorescence intensity and a number of cells; and
analyzing the histogram and performing a diagnosis of a tumor in the target sample,
wherein the performing the diagnosis comprises:
detecting an S phase fluorescence intensity range corresponding to S phase cells based on the fluorescence intensity corresponding to a peak of the number of cells in the histogram,
detecting an abnormal fluorescence intensity range corresponding to tumor cells in which DNA aneuploidy has occurred, based on a change in the number of cells in the S phase fluorescence intensity range, and
performing the diagnosis of the tumor in the target sample based on an index of the number of cells in a modified range defined by excluding the abnormal fluorescence intensity range from the S phase fluorescence intensity range.
2. The tumor diagnostic apparatus according to claim 1 , wherein the diagnosis of the tumor is a determination of a type of the tumor or a determination of malignancy of the tumor.
3. The tumor diagnostic apparatus according to claim 1 , wherein analyzing the histogram compares a representative value of the number of cells in the modified range with a value of the peak in the histogram to perform the diagnosis of the tumor in the target sample.
4. The tumor diagnostic apparatus according to claim 3 , wherein the representative value is a mean value, a mode value, or a median value.
5. The tumor diagnostic apparatus according to claim 1 , wherein the determination section compares an area under a curve in the modified range with an area under a curve in an entire range of the histogram to perform the diagnosis of the tumor in the target sample.
6. The tumor diagnostic apparatus according to claim 1 , wherein the determination section compares an area under a curve in the modified range with an area under a curve in a range defined by excluding a range corresponding to debris from an entire range of the histogram to perform the diagnosis of the tumor in the target sample.
7. A tumor diagnostic method comprising:
generating a histogram from a result of a measurement of fluorescence intensity of a suspension, the suspension having been produced from a target sample and comprising a cell containing DNA that is fluorescently stained, the histogram being indicative of a relation between the fluorescence intensity and a number of cells; and
performing a diagnosis of a tumor in the target sample by analyzing the histogram,
wherein the performing the diagnosis comprises:
detecting an S phase fluorescence intensity range corresponding to S phase cells based on the fluorescence intensity corresponding to a peak of the number of cells in the histogram;
detecting an abnormal fluorescence intensity range corresponding to tumor cells in which DNA aneuploidy has occurred, based on a change in the number of cells in the S phase fluorescence intensity range; and
performing the diagnosis of the tumor in the target sample based on an index of the number of cells in a range defined by excluding the abnormal fluorescence intensity range from the S phase fluorescence intensity range.
8. A non-transitory computer readable medium storing a program which, when executing on a computer, causes the computer to execute a method comprising:
generating a histogram from a result of a measurement of fluorescence intensity of a suspension, the suspension having been produced from a target sample and comprising a cell containing DNA that is fluorescently stained, the histogram being indicative of a relation between the fluorescence intensity and a number of cells; and
performing a diagnosis of a tumor in the target sample by analyzing the histogram,
wherein the performing the diagnosis comprises:
detecting an S phase fluorescence intensity range corresponding to S phase cells based on the fluorescence intensity corresponding to a peak of the number of cells in the histogram;
detecting an abnormal fluorescence intensity range corresponding to tumor cells in which DNA aneuploidy has occurred, based on a change in the number of cells in the S phase fluorescence intensity range; and
performing the diagnosis of the tumor in the target sample based on an index of the number of cells in a range defined by excluding the abnormal fluorescence intensity range from the S phase fluorescence intensity range.