IP Library Granted Patent US 10,633,715
Granted Patent B2
US 10,633,715 · App. 15/264,344 · Granted Apr 28, 2020

Gene controlling shell phenotype in palm

Inventors: Rajinder Singh (Kuala Lumpur, MY); Leslie Low Eng Ti (Kuala Lumpur, MY); Leslie Ooi Cheng Li (Kuala Lumpur, MY); Meilina Ong Abdullah (Seremban, MY); Rajanaidu Nookiah (Kuala Lumpur, MY); Ravigadevi Sambanthamurthi (Selangor, MY); Steven W. Smith (Fitchburg, WI); Nathan D. Lakey (Chesterfield, MO); Rob Martienssen (Cold Spring Harbor, NY); Jared Ordway (St. Louis, MO); Michael Hogan (Ballwin, MO)
Assignee: The Malaysian Palm Oil Board
C12Q1/6895A01H1/04C07K14/415C12N15/8261C12Q2600/13C12Q2600/156C12Q2600/158
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Quick Facts
Patent No.
US 10,633,715
App. No.
15/264,344
Granted
Apr 28, 2020
Kind
B2
Abstract

Nucleic acid sequences for predicting and controlling shell phenotype in palm.

Claims (13)

1. A method for segregating a plurality of E. guineensis palm plants or seeds into different shell phenotype categories, the method comprising:

obtaining the genomic sequence of each palm plant or seed at a polymorphic marker by conducting an assay, wherein the polymorphic marker is in a genomic region corresponding to SEQ ID NO:8218, thereby determing the genotype of the plant or seed at the polymorphic marker,

wherein heterozygosity at the polymorphic marker indicates a tenera shell phenotype, and

segregating the plants or seeds into groups based on the genotype at the polymorphic marker, wherein at least one group contains one of (i) plants or seeds that are heterozygous at the polymorphic marker, or (ii) plants or seeds that are homozygous at the polymorphic marker.

2. The method of claim 1 , wherein the plants or seeds are generated from (i) an attempted cross between a plant having the dura shell phenotype and a plant having the pisifera shell phenotype, (ii) selfing of a tenera palm, (iii) cross between two plants having the tenera shell phenotype, (iv) cross between dura and tenera palms, or (v) cross between tenera and pisifera palms.

3. The method of claim 1 , wherein the plants or seeds are 0-5 years old.

4. The method of claim 1 , wherein the plants or seeds are between zero and one year old.

5. The method of claim 1 , wherein the polymorphic marker is within a position in the genomic region corresponding to SEQ ID NO: 8219.

6. The method of claim 1 , wherein the polymorphic marker is within 1000 kb from a position in the genomic region corresponding to SEQ ID NO: 8219.

7. The method of claim 1 , further comprising selecting plants or seeds for cultivation if the plants or seeds are heterozygous at the polymorphic marker.

8. The method of claim 1 , further comprising selecting plants or seeds for cultivation if the plants or seeds are homozygous at the polymorphic marker.

9. The method of claim 1 , wherein the plants or seeds are discarded if the plants or seeds do not have a genotype predictive of the tenera shell phenotype.

10. The method of claim 1 , wherein the plants or seeds are discarded it the plants or seeds are not heterozygous at the polymorphic marker, wherein heterozgosity at the polymorphic marker indicates the tenera shell phenotype.

Assignments (3)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 11, 2018
From: SMITH, STEVEN W.; LAKEY, NATHAN D.; MARTIENSSEN, ROB; ORDWAY, JARED; HOGAN, MICHAEL
To: ORION GENOMICS
Reel/Frame 045778/0101 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 11, 2018
From: SINGH, RAJINDER; TI, LESLIE LOW ENG; LI, LESLIE OOI CHENG; ABDULLAH, MEILINA ONG; NOOKIAH, RAJANAIDU; SAMBANTHAMURTHI, RAVIGADEVI
To: MALAYSIAN PALM OIL BOARD
Reel/Frame 045778/0292 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 11, 2018
From: ORION GENOMICS
To: THE MALAYSIAN PALM OIL BOARD
Reel/Frame 046136/0605 →
Continuity (3)
Division 13800652 · Mar 13, 2013
Provisional Application 61612885 · Mar 19, 2012
Related Publication 20160376669A1 · Dec 29, 2016