Sample pre-compression valve for liquid chromatography

1. An injection valve for high pressure liquid chromatography, the injection valve comprising:

(a) a stator; and

(b) a rotor, in which a face surface of the rotor is configured to be rotated against a face surface of the stator, in which the stator comprises a first port, a second port, a third port, a fourth port, and a fifth port, the rotor comprises a first groove and a second groove, in which the first groove comprises a hook-shape, in which the first groove is configured to connect to two of the ports of the stator, and in which the second groove is configured to connect to a different two of the ports of the stator,

(i) in a LOAD position of the injection valve, the first groove connects the first port and the second port such that the first groove does not have a dead volume, and the second groove connects to the third port and the fourth port;

(ii) in a PRESSURE COMPENSATION position of the injection valve, the first groove connects the first port and the second port such that the first groove does have the dead volume, and the second groove does not connect the third port and the fourth port; and

(iii) in an INJECT position of the injection valve, the first groove connects the first port and the fifth port such that the first groove does not have the dead volume, and the second groove connects the second port and the third port.

2. The injection valve of claim 1 , in which the hook-shape comprises a first section and a second section, the first section connects a center of the rotor with a point of a circular path of the rotor, the second section is arranged on the circular path.

3. The injection valve of claim 2 , in which the second section comprises an arched shape.

4. The injection valve of claim 2 , in which the second section comprises a line shape.

5. The injection valve of claim 2 , in which the second groove comprises an arched groove configured to connect two points on the circular path of the rotor.

6. The injection valve of claim 2 , in which the second groove comprises a line-shaped groove configured to connect two points on the circular path of the rotor.

7. The injection valve of claim 1 , in which the first and second ports are arranged on a circular path around a center of the stator.

8. The injection valve of claim 7 , wherein the third, the fourth, and the fifth ports are arranged on the circular path around the center of the stator, in which the stator further includes a sixth port that is arranged at the center of the stator.

9. The injection valve of claim 8 , wherein the rotor further includes a third groove configured to connect the sixth port with one of the first port, the second port, the third port, the fourth port, and the fifth port.

10. The injection valve of claim 9 , wherein in the LOAD position, the third groove connects the fifth port and the sixth port such that the third groove does not have a dead volume; wherein in the PRESSURE COMPENSATION position, the third groove connects the fifth port and the sixth port such that the third groove does have the dead volume; and wherein in the INJECT position, the third groove connects the fourth port and the sixth port such that the third groove does not have the dead volume.

11. The injection valve of claim 10 , wherein in a PUMP PURGE position of the injection valve, the first groove connects the fourth port and the fifth port such that the first groove does not have the dead volume, the second groove connects the first port and the second port, and the third groove connects the third port and the sixth port such that the third groove does not have the dead volume.

12. The injection valve of claim 1 , in which the first port is arranged at a center of the stator and the second port is on a circular path around the center of the stator.

13. The injection valve of claim 12 , wherein the third, the fourth, and the fifth ports are arranged on the circular path around the center of the stator.

14. The injection valve of claim 1 , wherein in the LOAD position and the PRESSURE COMPENSATION position, the fifth port is not connected to the first groove, and the fifth port is not connected to the second groove.

15. The injection valve of claim 1 , wherein in the PRESSURE COMPENSATION position and the INJECT position, the fourth port is not connected to the first groove, and the fifth port is not connected to the second groove.

16. The injection valve of claim 1 , in which the first port, the second port, the third port, the fourth port, and the fifth port each have a point-shape or a groove-shape.

17. A method of injecting a sample into a chromatography column, the method comprising: pre-compressing the sample with an injection valve, the injection valve comprising:

(a) a stator; and

(b) a rotor, in which a face surface of the rotor is configured to be rotated against a face surface of the stator, in which the stator comprises a first port, a second port, a third port, a fourth port, and a fifth port, the rotor comprises a first groove and a second groove, in which the first groove comprises a hook-shape, in which the first groove is configured to connect to two of the ports of the stator, and in which the second groove is configured to connect to a different two of the ports of the stator,

(i) in a LOAD position of the injection valve, the first groove connects the first port and the second port such that the first groove does not have a dead volume, and the second groove connects to the third port and the fourth port;

(ii) in a PRESSURE COMPENSATION position of the injection valve, the first groove connects the first port and the second port such that the first groove does have the dead volume, and the second groove does not connect the third port and the fourth port; and

(iii) in an INJECT position of the injection valve, the first groove connects the first port and the fifth port such that the first groove does not have the dead volume, and the second groove connects the second port and the third port.

18. A sampler for liquid chromatography comprising:

(A) an injection valve including:

(a) a stator; and

(b) a rotor, in which a face surface of the rotor is configured to be rotated against a face surface of the stator, in which the stator comprises a first port, a second port, a third port, a fourth port, a fifth port, and a sixth port, the rotor comprises a first groove and a second groove, in which the first groove comprises a hook-shape, in which the first groove is configured to connect to two of the ports of the stator, and in which the second groove is configured to connect to a different two of the ports of the stator,

(i) in a LOAD position of the injection valve, the first groove connects the first port and the second port such that the first groove does not have a dead volume, and the second groove connects to the third port and the fourth port;

(ii) in a PRESSURE COMPENSATION position of the injection valve, the first groove connects the first port and the second port such that the first groove does have the dead volume, and the second groove does not connect the third port and the fourth port; and

(iii) in an INJECT position of the injection valve, the first groove connects the first port and the fifth port such that the first groove does not have the dead volume, and the second groove connects the second port and the third port;

wherein the first and the second ports are arranged on one or more circular paths around a center of the stator, in which the sixth port is arranged at the center of the stator;

(B) a sample conveying system comprising a syringe and a piston configured to be guided pressure-tight and moveable within the syringe;

(C) a sample line;

(D) a sample loop;

(E) a solvent pump; and

(F) a chromatography column, wherein the first port is connected to the fourth port via the sample loop, the second port is connected to the sample conveying system, the third port is connected with the sample line, the fifth port is connected to the chromatography column, and the sixth port is connected to the solvent pump.

19. A sampler for liquid chromatography comprising:

(A) an injection valve including:

(a) a stator; and

(b) a rotor, in which a face surface of the rotor is configured to be rotated against a face surface of the stator, in which the stator comprises a first port, a second port, a third port, a fourth port, and a fifth port, the rotor comprises a first groove and a second groove, in which the first groove comprises a hook-shape, in which the first groove is configured to connect to two of the ports of the stator, and in which the second groove is configured to connect to a different two of the ports of the stator,

(i) in a LOAD position of the injection valve, the first groove connects the first port and the second port such that the first groove does not have a dead volume, and the second groove connects to the third port and the fourth port;

(ii) in a PRESSURE COMPENSATION position of the injection valve, the first groove connects the first port and the second port such that the first groove does have the dead volume, and the second groove does not connect the third port and the fourth port; and

(iii) in an INJECT position of the injection valve, the first groove connects the first port and the fifth port such that the first groove does not have the dead volume, and the second groove connects the second port and the third port;

(B) a sample loop including a sample conveying system and a needle seat, the sample conveying system comprising a syringe and a piston configured to be guided pressure-tight and moveable within the syringe;

(C) a discharge line;

(D) a solvent pump; and

(E) a chromatography column, wherein the first port is connected to the solvent pump, the second port is connected to the chromatography column, the third port and the fifth port are connected to the sample loop, and the fourth port is connected to the discharge line.